树突状细胞亚型变化在儿童咳嗽变应性哮喘中的临床意义
| 作者: |
1王华,
1许志有,
1林鲁飞,
1张余转
1 海口市第三人民医院儿科，海口 571100 |
| 通讯: |
王华
Email: 13637624799@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.10.013 |
| 基金: | 海南省自然科学基金 (817391)。 |
摘要
目的：观察咳嗽变异性哮喘(cough var iant asthma，CVA)儿童外周血树突状细胞mDCs和pDCs两种亚型的变化，并探讨其临床意义。方法：收集海口市第三人民医院2017年3月至2018年4月收治的C VA儿童62例。另取同期健康体检的儿童40例作为对照。用流式细胞仪检测外周血mDCs和pDCs的比例，ELISA法检测外周血血浆中I FN-γ和I L -4表达。结果：对照组和CVA组儿童mDCs间差异无统计学意义(P>0.05)，CVA组儿童pDCs为(0.92±0.24)%，显著高于对照组的(0.31±0.08)%(P<0.01)，mDCs/pDCs比值在CVA组儿童为0.62±0.15，显著低于对照组的1.68±0.34(P<0.01)。CVA组儿童IFN-γ和IFN-γ/IL -4比值分别为(40.47±8.45) ng/mL和0.92±0.14，显著低于对照组的(53.36±10.42) ng/mL和2.51±0.46(P<0.05和P<0.01)。CVA组儿童IL -4为(43.81± 7.16) ng/mL，显著高于对照组的(21.27±4.15) ng/mL(P<0.01)。结论：CVA儿童外周血pDCs显著升高，导致了患儿体内的Thl/Th2失衡，pDCs有可能成为CVA诊断和治疗的新靶点。
关键词：
咳嗽变异性哮喘；儿童；髓样树突状细胞；浆细胞样树突状细胞；Thl/Th2平衡
Clinical significance of subtypes of dendritic cells in children with cough allergic asthma
CorrespondingAuthor: WANG Hua Email: 13637624799@163.com
DOI: 10.3978/j.issn.2095-6959.2018.10.013
Foundation: This work was supported by the Natural Science Foundation of Hainan Province, China (817391).
Abstract
Objective: To detect the changes of mDCs and pDCs subtypes in peripheral blood of children with cough variant asthma (CVA). Methods: Sixty-two CVA and 40 healthy children were enrolled in this study. The mDCs and pDCs was detected by flow cytometry. IFN-γ and IL-4 was detected by ELISA analysis. Results: There was no significant difference of mDCs between CVA and healthy children (P>0.05). The pDCs was (0.92±0.24)% in CVA children, which was higher than that of (0.31±0.08)% in healthy children (P<0.01). The mDCs/pDCs was 0.62±0.15 in CVA children, which was lower than that of 1.68±0.34 in healthy children (P<0.01). The IFN-γ and IFN-γ/ IL-4 was (40.47±8.45) ng/mL and 0.92±0.14 in CVA children, which was lower than that of (53.36±10.42) ng/mL and 2.51±0.46 in healthy children (P<0.05 and P<0.01). The IL -4 was (43.81±7.16) ng/mL in CVA children, which was higher than that of (21.27±4.15) ng/mL in healthy children (P<0.01). Conclusion: The pDCs in peripheral blood is increased significantly, which causes Thl/Th2 imbalance in CVA children.
Keywords：
cough variant asthma; children; myeloid dendritic cells; plasmacytoid dendritic cells; Thl/Th2 balance