长链非编码RNA-HULC 调控肺腺癌细胞的增殖和侵袭
| 作者: |
1王鹏,
1李青
1 徐州市中心医院检验科，江苏 徐州 221009 |
| 通讯: |
李青
Email: phoenix010149@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.06.007 |
摘要
目的：探讨长链非编码RNA-HULC(long non-coding RNA-HULC，lncRNA-HULC)对肺腺癌细胞增殖和侵袭过程的调控作用。方法：收集57例肺癌患者的癌组织及与其配对的癌旁组织，通过实时定量PCR(quantitative real-time PCR，qRT-PCR)法检测组织及细胞中lncRNA-HULC的表达水平；采用RNA干扰技术(siRNA)下调lncRNA-HULC的表达，同时以qRT-PCR法检测其干扰效率。CCK-8法和Transwell法检测下调lncRNA-HULC对肺腺癌细胞增殖和侵袭过程的影响。结果：与癌旁组织相比，lncRNA-HULC在癌组织中的表达显著升高；与正常肺上皮细胞相比，lncRNA-HULC在肺癌细胞中的表达也显著升高。下调lncRNA-HULC的表达后，肺腺癌细胞的增殖和侵袭能力均降低。结论：下调lncRNA-HULC可抑制肺腺癌细胞的增殖和侵袭过程。
关键词：
肺癌；长链非编码RNA-HULC；细胞增殖；细胞侵袭
Down-regulation of long non-coding RNA-HULC suppresses cell proliferation and invasion in lung adenocarcinoma
CorrespondingAuthor: LI Qing Email: phoenix010149@163.com
DOI: 10.3978/j.issn.2095-6959.2018.06.007
Abstract
Objective: To investigate the potential molecular mechanism of long non-coding RNA-HULC (lncRNA-HULC) in the proliferation and invasion of lung adenocarcinoma cell lines. Methods: Quantitative RT-PCR was used to detect the expression of lncRNA-HULC in lung cancer tissues and cell lines. Small interfering RNA (siRNA) was used to down-regulate lncRNA-HULC expression. CCK-8 assay and Transwell assay were performed to explore cell proliferation and invasion. Results: Compared with adjacent tissues, the expression of lncRNA-HULC was increased in lung cancer tissues. Compared with normal lung epithelial cell, lncRNA-HULC was also increased in lung cancer cell lines. Silence of lncRNA-HULC inhibited cell lines proliferative and invasive ability. Conclusion: Down-regulation of lncRNA-HULC can cell proliferation and invasion in suppress lung adenocarcinoma.
Keywords：
lung cancer; long non-coding RNA-HULC; cell proliferation; cell invasion