Objective: To observe the effect of lncRNA NEAT1 on cell proliferation, invasion and migration in hepatocellular cell lines, and investigate the mechanism of it. Methods: qRT-PCR was set out to detect the expression pattern of lncRNA NEAT1 in normal hepatocyte line, L02, and hepatocellular cell lines, HepG2, SMMC-7721 and Huh7. The HepG2 cell line was divided into a NEAT1-siRNA group, a Control-siRNA group and a Mock group, which was transfect with pcDNA3.1-lncRNA-NEAT1-shRNA, pcDNA3.1-lncRNA-NEAT1-NC and PBST by Lipofectamine^TM 2000. CCK-8 assay, cell wound scratch and transwell assay was used to test the proliferation, migration and invasion ability. The expression level of miR-121 and SATB2 protein was measured by qRT-PCR and Western blot. Results: LncRNA NEAT1 expression level was significantly higher in hepatocellular cell lines, HepG2, SMMC-7721 and Huh7, than normal hepatocyte cell line L02 (P<0.05). CCK-8 showed that OD_{450 nm} of NEAT1-siRNA group was significantly lower than that in the Control-siRNA group and the Mock group after transfect for 72 and 96 h (P<0.05). The scratch healing rate of the NEAT1-siRNA group was significantly lower than that in the Control-siRNA group [(30.38±4.19)% vs (54.78±5.83)%, P<0.05]. The invasive cell number of NEAT1-siRNA group was significantly lower than that in the Control-siRNA group [(107.5±8.1) vs (178.1±13.4), P<0.05]. The expression level of miR-211 in the NEAT1-siRNA group was significantly higher than that in the Control-siRNA group, the expression level of SATB2 protein was significantly lower than that in Control-siRNA group (P<0.01). Conclusion: LncRNA NEAT1 is high expressed in hepatocellular cell lines. Silencing expression of lncRNA NEAT1 can inhibit proliferation, migration and invasion of hepatocellular cells, which mechanism may be associated with miR-211/SATB2 cell signaling.