姜黄素对慢性低灌注大鼠的行为学功能保护作用
| 作者: |
1周婷,
2李青叶,
1黄菲菲,
1候明
1 武汉市东西湖区人民医院神经内科，武汉 430040 2 湖北省中医院神经内科，武汉 430040 |
| 通讯: |
周婷
Email: zhouting1230@yeah.net 李青叶 Email: zhouting1230@yeah.net 黄菲菲 Email: zhouting1230@yeah.net 候明 Email: zhouting1230@yeah.net |
| DOI: | 10.3978/j.issn.2095-6959.2015.06.022 |
摘要
目的：探讨姜黄素在慢性低灌注大鼠的神经功能保护作用。方法：制备慢性脑缺血模型(2-VO)模型，术后分别给予姜黄素(实验组)和磷酸盐缓冲液(PBS，对照组)干预。术后8周后行Morris水迷宫评价大鼠的空间学习记忆功能变化。同时从Nissl染色观察大鼠神经细胞的变化；Western blot检测炎症因子IL-1β、TNF-α的表达。结果：实验组大鼠的空间学习记忆能力均较对照组明显改善，差异有统计学意义(P<0.05)；Nissl染色发现实验组大鼠的缺血区神经元细胞与形态相对较为完整，而对照组大鼠神经元形态不完整，仅有散在的尼氏小体；在姜黄素治疗后缺血区脑细胞中IL-1β、TNF-α的含量明显降低(P<0.05)。结论：姜黄素可以显著改善慢性脑缺血大鼠的空间学习记忆能力，可能与其抗炎症反应相关。
关键词：
姜黄素
脑缺血
水迷宫
炎性因子
神经保护
Experimental study of the curcumin in chronic cerebral ischemic rats
CorrespondingAuthor: ZHOU Ting Email: zhouting1230@yeah.net
DOI: 10.3978/j.issn.2095-6959.2015.06.022
Abstract
Objective: Explore the effect of curcunmin on chronic cerebral ischemia in rats, and its possible mechanism. Methods: Chronic cerebral ischemia (2-VO) model were prepared and given curcunmin or PBS after the surge. Eight weeks after the operation, Morris water maze were carried out to evaluate the learning and memory ability of the rats. The change of nerve cells were observed by Nissl staining; the expression of IL-1β, TNF-α were detected by western blot. Results: The learning and memory ability of the curcunmin group were significantly improved compared with the control group. The neurons pattern of curcumin treatment group was similar to the control group, while only scattered Nissl bodies were found the curcumin group; the expression of IL-1β, TNF-α in curcumin treatment group were significantly reduced (P<0.05). Conclusion: Curcumin can significantly improve the learning and memory ability of the chronic cerebral ischemic rat and its possible mechanism maybe involve in the anti-inflammation in neurons.