过表达细胞黏附分子1抑制卵巢癌细胞迁移及侵袭
| 作者: |
1王晓凤,
1柳艳飞,
1田勇,
1何为,
1金红艳
1 武汉科技大学附属武汉市普仁医院肿瘤科，武汉 430000 |
| 通讯: |
金红艳
Email: zzh2016778@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.01.006 |
摘要
目的：研究过表达细胞黏附分子1(cell adhesion molecule 1，CADM1)对卵巢癌增殖、迁移和侵袭的影响。方法：qRT-PCR测定CADM1mRNA在卵巢癌细胞系SKOV3及人正常卵巢上皮细胞hose 中的表达，将SKOV3细胞分成两组，即CADM1过表达组和对照组，转染48 h后Western印迹测定两组CADM1蛋白表达量，采用lipofectamine 2000分别转染pcDNA3.1-CADM1及pcDNA3.1质粒，采用CCK-8、克隆形成、细胞划痕及Transwell实验分别检测两组细胞增殖、克隆形成、细胞迁移及侵袭能力。结果：CADM1mRNA在SKOV3中表达水平显著低于hose细胞系(1.54±0.34 vs. 5.63±0.96，P<0.05)；转染48 h后，CADM1过表达组和对照组CADM1蛋白表达量分别为2.53±0.42，0.37±0.09，差异具有统计学意义(P<0.05)。CADM1过表达组和对照组在0，24，48，72 h 450 nm处的OD值差异无统计学意义(P>0.05)；CADM1过表达组与对照组克隆形成数相比(60.4±7.6 vs. 58.3±8.2)，差异无统计学意义(P>0.05)；CADM1过表达组细胞迁移率显著低于对照组(20.3%±3.5% vs. 60.1%±4.2%，P<0.05)；CADM1过表达组侵袭细胞数显著少于对照组(24.5±5.3 vs. 65.1±6.9，P<0.05)。结论：CADM1在卵巢癌细胞系中低表达，过表达CADM1对卵巢癌细胞增殖和克隆形成无影响，但可抑制迁移和侵袭，起抑癌基因的作用。
关键词：
细胞黏附分子1
卵巢癌
迁移
侵袭
Overexpression of cell adhesion molecule 1 inhibits the migration and invasion of ovarian carcinoma
CorrespondingAuthor: JIN Hongyan Email: zzh2016778@sina.com
DOI: 10.3978/j.issn.2095-6959.2017.01.006
Abstract
Objective: To investigate the effect of over expression of cell adhesion molecule 1 (CADM1) on cell proliferation, migration and invasion of ovarian carcinoma. Methods: The expression level of CADM1 mRNA of SKOV3 and hose was measured by qRT-PCR. The SKOV3 cell line was divided into two groups, CADM1 over-expression group was transfected with pcDNA3.1-CADM1 plasmid, and control group with pcDNA3.1 plasmid by lipofectamine 2000. The expression level of CADM1 of those two groups was measured by Western Blot. The proliferation, clone formation, migration and invasive ability was measured and compared between those two groups by CCK-8, clone formation, cell scratch-wound and Transwell assay respectively. Results: The expression level of CADM1mRNA was 1.54±0.34 in SKOV3 cell line and 5.63±0.96 in hose normal cell line, the difference was statistically significant (P<0.05). The protein expression level in CADM1 over-expression group and control group was 2.53±0.42 and 0.37±0.09 respectively after transfected for 48 h, the difference was statistically significant (P<0.05). There was no significant difference between those two groups in terms of OD value of 450 nm in 0, 24, 48 and 72 h (P>0.05). The number of clone cells was 60.4±7.6 in CADM1 over-expression group, which was higher than that (58.3±8.2) in control group, the difference has no statistical significance (P>0.05). The cell migration rate of CADM1 over-expression group was significantly lower than control group (20.3%±3.5% vs. 60.1%±4.2%, P<0.05). The invasive cell number of CADM1 over-expression group was significantly lower than control group (24.5±5.3 vs. 65.1±6.9, P<0.05). Conclusion: CADM1 showed low expression in ovarian carcinoma cell line. Over-expression of CADM1 had no influence on proliferation and clone formation in ovarian carcinoma cell line, while it inhibits the migration and invasive ability which demonstrates that CADM1 was a tumor suppressor.