非小细胞肺癌胸水细胞块c-MET基因扩增检测分析
| 作者: |
1朱礼阳,
2徐建平,
1于忠和,
2赵洁婷,
2叶伟,
2朱东波,
2孙晓,
2宋蓉蓉,
3许春伟
1 陆军总医院肿瘤科，北京 100700 2 安徽省胸科医院病理科，合肥 230032 3 福建省肿瘤医院病理科，福州 350014 |
| 通讯: |
于忠和
Email: 773080192@qq.com 许春伟 Email: xuchunweibbb@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2016.11.013 |
摘要
目的：探讨非小细胞肺癌胸水细胞块在间充质上皮转化因子受体(c-mesenchymal-epithelial transition，c-MET)基因扩增检测中的临床价值。方法：采用RT-PCR法检测215例非小细胞肺癌细胞块和404例非小细胞肺癌组织块中c-MET基因扩增，并检测细胞块同时送检组织块的患者74例的一致性。结果：细胞块c-MET基因扩增31例，扩增率14.42%(31/215)；组织块c-MET基因扩增35例，扩增率8.66%(35/404)；74例有组织块对照的细胞块c-MET结果一致性有68例，一致率达91.89%(68/74)，其中细胞块c-MET基因扩增率12.16%(9/74)，组织块扩增率17.57%(13/74)。结论：非小细胞肺癌胸水细胞块c-MET的扩增率略高于组织块；有恶性胸水的非小细胞肺癌患者原发灶组织发生c-MET扩增的概率较高。
关键词：
肺肿瘤
非小细胞肺癌
细胞块
c-MET
Analysis of cell blocks c-MET amplification in pleural effusion of non-small cell lung cancer
CorrespondingAuthor: YU Zhonghe Email: 773080192@qq.com
DOI: 10.3978/j.issn.2095-6959.2016.11.013
Abstract
Objective: To investigate the clinical value of the cell blocks for gene amplification detection of c-mesenchymal-epithelial transition (c-MET). Methods: Two hundred and fifteen cases of cell block from pleural effusion of non-small cell lung cancer (NSCLC) were collected. Four hundred and four cases of tissue block were detected by RT-PCR method. The consistency of c-MET amplification was examined in 74 cases of patients with tissue block and cell block. Results: c-MET amplification was found in 31 of 215 cell blocks (positive detection rate of 14.42%). c-MET amplification was detected in 35 of 404 tissue blocks (positive detection rate of 8.66%). There were 68 cases in the 74 (91.89%) cases had the same consistency as tissue block. c-MET amplification was detected in 9 of 74 (12.16%) cell blocks, and 13 of 74 (17.57%) tissue blocks. Conclusion: The rate of c-MET amplification in cell blocks of non-small cell lung cancer is higher than in matched tissue blocks. The patients with malignant pleural effusion are likely to tend c-MET amplification.