EGFR 19-Del上调Fn14及JAK/STAT的表达促进非小细胞肺癌的发生发展
| 作者: |
1冯征,
1孙盈,
1周勇安,
1王雪娇,
1文苗苗,
1夏靖华,
1邢昊,
1王磊,
1李小飞,
1张志培
1 第四军医大学唐都医院胸腔外科，西安 710038 |
| 通讯: |
张志培
Email: zzpzyy@fmmu.edu.cn |
| DOI: | 10.3978/j.issn.2095-6959.2016.11.005 |
摘要
目的：本研究检测Fn14、p-JAK1、p-STAT1在EGFR 19-Del的非小细胞肺癌(non-small cell lung cancer，NSCLC)细胞系中的表达，并探讨EGFR 19-Del对Fn14及JAK1/STAT1表达的调控作用，以期为EGFR 19-Del之NSCLC的发展机制研究奠定基础。方法：采用EGFR TKI(吉非替尼)处理HCC827细胞系(EGFR 19-Del)、H1975(L858R)及H292细胞系(正常肺上皮细胞)，Western blot法检测Fn14、p-JAK1、p-STAT1蛋白表达。结果：相比于H1975和H292细胞系，Fn14、p-JAK1及p-STAT1基因和蛋白在HCC827细胞系中有较高的表达水平。对比未经吉非替尼处理的HCC827细胞系，经抑制剂处理后的HCC827细胞系中Fn14、p-JAK1、p-STAT1蛋白表达明显受到抑制，而在H292细胞系中无此现象。由此说明，EGFR 19-Del对Fn14及JAK/STAT信号分子的表达具有调控作用。结论：EGFR 19-Del可能通过上调Fn14及JAK/STAT信号分子的表达促进NSCLC的发生发展，而Fn14可能是EGFR 19-Del之NSCLC潜在的治疗靶点。
关键词：
Fn14
EGFR 19缺失突变
JAK/STAT
非小细胞肺癌
EGFR 19-Del up-regulate Fn14, JAK/STAT expression to promote non-small cell lung cancer tumorigenesis
CorrespondingAuthor: ZHANG Zhipei Email: zzpzyy@fmmu.edu.cn
DOI: 10.3978/j.issn.2095-6959.2016.11.005
Abstract
Objective: To investigate the basic role of EGFR 19-Del in the progression of non-small cell lung cancer (NSCLC), the expression of Fn14, p-JAK1 and p-STAT1 were detected in NSCLC cell lines. Methods: The protein expression of Fn14, p-JAK1, p-STAT1 were detected using Western blot in HCC827, H1975 and H292 cell lines before or after EGFR TKI treatment. Results: Contrast to H1975 and H292 cell lines, the gene and protein expression of Fn14, p-JAK1, p-STAT1 were higher in HCC827 cell lines. The results showed that EGFR-TKI (gefitinib) can reduce Fn14, p-JAK1, p-STAT1 protein expression in HCC827 cell lines, but not in H292 cell lines. Conclusion: EGFR 19-Del might up-regulate Fn14, JAK/STAT expression to promote NSCLC carcinogenesis, and Fn14 maybe a new potential therapeutic target in NSCLC with EGFR 19-Del.