文章摘要

恶性黑色素瘤患者肿瘤组织中BRAF基因突变的分子病理检测

作者: 1田玉旺, 2许春伟, 1张立英, 3方美玉, 4沈斌
1 陆军总医院病理科，北京 100700
2 福建省肿瘤医院病理科，福州 350014
3 浙江省肿瘤医院综合肿瘤内科，杭州 310022
4 浙江省湖州市中心医院介入放疗科，浙江 湖州 313000
通讯: 许春伟 Email: xuchunweibbb@163.com
方美玉 Email: fangjade2004@icloud.com
DOI: 10.3978/j.issn.2095-6959.2017.03.024
基金: 浙江省卫生科研计划基金, 2013KYB051 浙江省中医药局科研基金, 2013ZQ005

摘要

目的：探讨恶性黑色素瘤患者肿瘤组织中BRAF基因突变情况。方法：应用Taqman-ARMS方法检测164例恶性黑色素瘤患者肿瘤组织中BRAF基因突变情况。结果：恶性黑色素瘤患者肿瘤组织中BRAF基因总突变率为11.59%(19/164)，均为V600E突变，BRAF基因突变在黑色素瘤亚型和临床分期Ⅰ或Ⅱ中差异有统计学意义(P<0.05)。结论：恶性黑色素瘤患者中BRAF基因存在一定的突变率，且慢性日光损伤型或非慢性日光损伤型及Ⅲ或Ⅳ期恶性黑素瘤BRAF基因突变多见。
关键词： Taqman-ARMS方法 恶性黑色素瘤 BRAF基因

Molecular pathology detection of BRAF gene mutation in malignant melanoma

Authors: 1TIAN Yuwang, 2XU Chunwei, 1ZHANG Liying, 3FANG Meiyu, 4SHEN Bin
1 Department of Pathology, Army General Hospital, Beijing 100700
2 Department of Pathology, Fujian Cancer Hospital, Fujian Medical University Cancer Hospital, Fuzhou 350014
3 Department of Comprehensive Medical Oncology, Zhejiang Cancer Hospital, Hangzhou 310022
4 Department of Interventional Radiation Oncology, Huzhou Central Hospital, Huzhou Zhejiang 313000, China

CorrespondingAuthor: XU Chunwei Email: xuchunweibbb@163.com

DOI: 10.3978/j.issn.2095-6959.2017.03.024

Abstract

Objective: To investigate the mutations of BRAF gene in malignant melanoma. Methods: Taqman-ARMS was used to detect the tissues in 164 patients of malignant melanoma with paraffin tissue BRAF gene mutation. Results: The total BRAF gene mutation rate was 11.59% (19/164) in malignant melanoma, and they were all V600E mutation. BRAF gene mutation in melanoma subtype and clinical stage were statistically significant (P<0.05). Conclusion: BRAF gene mutation has a certain mutation rate in malignant melanoma patients, especially in chronic sun damage type or non-chronic sun damage type and III or IV stage.

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