非小细胞肺癌胸水细胞块ALK融合基因检测分析
| 作者: |
1朱礼阳,
2徐建平,
1于忠和,
2赵洁婷,
2叶伟,
2朱东波,
2孙晓,
2宋蓉蓉,
3许春伟
1 陆军总医院肿瘤科，北京 100700 2 安徽省胸科医院病理科，合肥 230032 3 福建省肿瘤医院病理科，福州 350014 |
| 通讯: |
于忠和
Email: 773080192@qq.com 许春伟 Email: xuchunweibbb@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2016.12.013 |
| 基金: | 国家自然科学基金, 81372489 北京市科技计划课题, 2131100006813032 |
摘要
目的：探讨非小细胞肺癌(non-small cell lung cancer，NSCLC)胸水细胞块在间变性淋巴瘤激酶(anaplastic lymphoma kinase，ALK)融合基因检测中的临床价值。方法：采用突变扩增系统PCR(ARMS-PCR)法检测215例NSCLC细胞块和404例NSCLC组织块中ALK基因的三类融合类型，并检测细胞块同时送检组织块的患者74例的一致性。结果：细胞块ALK基因融合阳性26例，阳性率12.09%(26/215)；组织块ALK基因融合阳性25例，阳性率6.19%(25/404)；74例有组织块对照的细胞块ALK融合基因结果一致性有67例，一致率达90.54%(67/74)，其中细胞块ALK融合基因的阳性率14.86%(11/74)，组织块阳性率18.92%(14/74)。结论：NSCLC胸水细胞块ALK融合基因的阳性率略高于组织块；有恶性胸水的NSCLC患者原发灶组织发生ALK融合基因阳性的概率较高。
关键词：
肺肿瘤
非小细胞肺癌
细胞块
间变性淋巴瘤激酶
Analysis of cell blocks ALK fusion gene in pleural effusion of non-small cell lung cancer
CorrespondingAuthor: YU Zhonghe Email: 773080192@qq.com
DOI: 10.3978/j.issn.2095-6959.2016.12.013
Abstract
Objective: To investigate the clinical value of the cell blacks for anaplastic lymphoma kinase (ALK) fusion gene detection in non-small cell lung cancer (NSCLC). Methods: 215 cases of cell block from pleural effusion of non-small cell lung cancer were collected. Three types of ALK fusion and 404 cases of tissue block were detected by ARMS-PCR method. The consistency of ALK fusion was examined in 74 cases of patients with tissue block and cell block. Results: ALK fusion was found in 26 of 215 cell blocks, the positive detection rate was 12.09%. ALK fusion was detected in 25 of 404 tissue blocks, the positive detection rate was 6.19%. 67 cases in the 74 (90.54%) cases had the same result as tissue block. ALK fusion was detected in 11 of 74 (14.86%) cell blocks, and 14 of 74 (18.92%) tissue blocks. Conclusion: The rate of ALK fusion in cell blocks of non-small cell lung cancer is higher than that in matched tissue blocks. The patients with malignant pleural effusion are likely to tend to ALK fusion.