Cardiovascular disease (CVD) is the leading cause of death worldwide. Hydrogen sulfide (H2S), as a gaseous signaling molecule, plays an important role in the development of CVD by promoting angiogenesis, stimulating vasodilation and preventing vascular endothelial cell senescence. Recent studies have shown that H2S can improve cardiac functions by regulating expressions of microRNAs and their bioactivities. miRNAs are a class of highly conserved, noncoding small RNA molecules found in eukaryotic cells, which regulate gene expression by inhibiting protein translation and accelerating mRNA degradation, and are expected to be intervention targets for CVD. Some miRNAs can also modulate the biosynthesis of H2S, and the mutual regulations between H2S and miRNAs open up a new pathway for exploration of CVD development and intervention.