TaqMan探针法与尿素呼气试验法检测胃镜活检标本幽门螺旋杆菌的比较
| 作者: |
1沈和德,
1褚明亮,
1易韦,
2杨文秀
1 贵州医科大学附属人民医院病理科，贵阳 550002 2 贵州医科大学病理学教研室，贵阳 550004 |
| 通讯: |
易韦
Email: yiwei6252@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.03.005 |
| 基金: | 国家自然科学基金, 81560088 |
摘要
目的：通过比较幽门螺旋杆菌(Helicobacter pylori，Hp)感染的两种检测方法，探讨TaqMan探针法在诊断幽门螺旋杆菌感染中的价值。方法：抽取70例有完整病例资料的胃镜活检标本，进行Hp分型、鉴定检测，并将实验结果与临床的碳14尿素呼气试验和病理结果进行相关性分析。结果：Hp感染的两种检测方法(TaqMan探针法和呼气实验法)一致性程度尚可(Kappa=0.550)，两种方法检测的阳性率都随病理炎症程度的加重而增加，而呼气实验法检测Hp的阳性率更高(P<0.05)。但TaqMan探针法可以将Hp进行不同毒力类型的鉴定，且TaqMan探针法可区分Hp的阳性强度(1+，2+，3+)，其与病理的炎症程度有较好的相关性(γ=0.564，P<0.05)。结论：TaqMan探针法可以定性地鉴定Hp，与呼气实验法有较好的一致性。TaqMan探针法可进一步定量Hp的阳性强度，更好地反映临床的病理炎症程度。
关键词：
幽门螺旋杆菌
TaqMan探针法
尿素呼气试验法
Comparison of TaqMan PCR method and Carbon-14-Urea breath test in the diagnosis of Helicobacter pylorus
CorrespondingAuthor: YI Wei Email: yiwei6252@sina.com
DOI: 10.3978/j.issn.2095-6959.2017.03.005
Abstract
Objective: To investigate and compare TaqMan PCR method and Carbon-14-Urea breath test on Helicobacter pylori (Hp) detection. Methods: TaqMan PCR method was used in 70 cases of clinical samples, and the results were analyzed. Results: There was no difference in the positive rate of Hp in TaqMan PCR method and Carbon-14-Urea breath test, and there was a good correlation between the two methods, but the positive rate was higher in Carbon-14-Urea breath test. Further study showed that the positive intensity of Hp can be divided into 3 types by TaqMan PCR method, and there was a good correlation between positive intensity and pathological degree of inflammation. Conclusion: TaqMan PCR method can be used as a useful molecular biological method for clinical diagnosis of Hp.