Objective: To study the effect of the mini-clinical evaluation exercise (mini-CEX) in prevention of needle-stick injuries in nursing students. Methods: 100 nursing students were numbered and divided into control group and experimental group, which has 50 students respectively. The control groups were using traditionally methods to assess clinical skills exam. Mini-CEX were applied in the experimental group to assess the practices of intravenous infusion, and to observe the occurrences of needle-stick injuries during the 8 months clinical practicing, which can also help to evaluate the affection of the Mini-CEX through our observation and nursing students’ feedback. Results: The incidence of needle-stick injuries in the experimental group was 10%, with a frequency of 0.1/(8 months), which was significantly lower than the control group 32% and 0.38/8 months. From the questionnaire, mini-CEX got a high recognition which was over 80%. Conclusion: The using of the mini-CEX in intravenous infusion can help to find the deficiency in occupational protection of nursing students, to standardize their performances, and to improve their vocational protection awareness.