文章摘要

睾丸孤核受体4(TR4)促进巨噬细胞向前列腺癌浸润

作者: 1刘鹏举, 1秦勇, 2朱进, 1刘渊, 1顾海, 1梅方, 1丁献凡
1 杭州市红十字会医院泌尿外科，杭州 310000
2 苏州大学附属第二医院泌尿外科，江苏 苏州 215000
通讯: 丁献凡 Email: xianfan023@sina.cn
DOI: 10.3978/j.issn.2095-6959.2016.10.011
基金: 杭州市卫生科技计划(一般)项目, 201450029

摘要

目的：研究前列腺癌TR4水平对巨噬细胞浸润的影响。方法：用transwell体系进行巨噬细胞THP-1迁移实验，在人体组织中研究睾丸孤核受体4(Testicular orphan receptor 4，TR4)水平和巨噬细胞标志物CD36的关系。结果：过表达TR4促进了巨噬细胞向前列腺的迁移；敲低TR4抑制了巨噬细胞向前列腺癌的迁移；人体前列腺癌组织标本中TR4较高的患者，CD36表达较高。结论：前列腺癌的TR4可能促进了巨噬细胞浸润。
关键词： 前列腺癌 睾丸孤核受体4 巨噬细胞 浸润

Testicular orphan receptor 4 (TR4) promotes human macrophage infiltration in prostate cancer

Authors: 1LIU Pengju, 1QIN Yong, 2ZHU Jin, 1LIU Yuan, 1GU Hai, 1MEI Fang, 1DING Xianfan
1 Department of Urology, Hangzhou Red Cross Hospital, Hangzhou 310000
2 Department of Urology, the Second Affiliated Hospital of Soochow University, Suzhou Jiangsu 215000, China

CorrespondingAuthor: DING Xianfan Email: xianfan023@sina.cn

DOI: 10.3978/j.issn.2095-6959.2016.10.011

Abstract

Objective: To investigate the role of testicular orphan receptor 4 (TR4) in the recruitment of human macrophage THP-1 cells in prostate cancer. Methods: Transwell system was used in the study of cellular migration to measure the THP-1 recruitment ability of prostate cancer (PCa) cells with varying levels of TR4; human PCa tissue was collected to study the relation of TR4 levels and CD36 levels by IHC staining. Results: Over-expression of TR4 increased THP-1 migration in PCa cells, knocking-down TR4 could decrease the THP-1 migration in PCa cells; IHC staining demonstrated that TR4 levels positively correlate with CD36 levels. Conclusion: TR4 may promote THP-1 cells infiltration in prostate cancer.

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