Objective: To detect the meaning of CXCL13, PD-1, CD10 and bcl-6 on the diagnosis of angioimmunoblastic T cell lymphoma (AITL). Methods: 27 cases of AITL retrieved from in-house and consultation files (from January 2009 to July 2015) were reviewed with emphasis on the clinicopathologic characteristics, immunophenotypes and the prognostic impact. Results: Immunophenotypes: CD3 (27/27), CXCL13 (27/27), PD-1 (27/27), CD10 (20/27), bcl-6 (23/27), CD30 focal positive in 3 cases (3/27), CD20 (0/27). In CD4 and CD8 positive AITL cases: 20 cases were CD4 dominantly expressed, 5 cases were CD8 dominantly expressed, one case was both CD4 and CD8 positive. The staining of CD21 showed follicular dendritic cell positive, and reveal the pattern with the cells arranged around the blood vessel, which prompting the exist of follicular dendritic in tumor cells. Conclusion: AITL is the type of highly invasive tumor and derived from helper T cells of germinal center. Application of CXCL13, PD-1, CD10 and bcl-6 immunostains offers a reasonable marker for diagnosis, and combination of the four antibodies can improve the standard of diagnosis. Moreover, the role of CD30 immunoreactivity can give a differentiation diagnosis in AITL, besides the clinical significance needs more research.