Objective: To explore the effect of all-in-one nursing intervention to hospital stay and anxiety of the patients cured by conservative treatment because of acute pancreatitis. Methods: Selected 80 cases of acute pancreatitis treated by conservative in our department of the hospital from February 2013 to February 2014. The patients were randomly divided into experiment group with all-in-one nursing intervention and control group with traditional fundamental nursing intervention, each 40 cases. The hospital stay, anxiety grade and patients’ satisfaction were compared and analyzed. Results: The hospital stay and anxiety grade of control group were observably higher than those of experiment group, but the degree of satisfaction of control group was observably lower than those of experiment group, the differences were statistically significant (P<0.05). Conclusion: All-in-one nursing intervention is helpful to shorten the hospital stay, remit anxiety and exchange degree of satisfaction for the patients with acute pancreatitis cured by conservative treatment. Also, it’s helpful to shape the hospital image in public and bring positive impact on the developing of hospital.