Objective: To study the influence and effect of chitosan on ocular surface function of dry eye patients. Methods: Used random sampling method to select 100 cases (200 eyes) patients with dry eye from August 2013 to August 2014 in our hospital, and conducted the research on the clinical data. Then randomly divided the dry eye patients into two groups, each with 50 cases (100 eyes). Observation group were treated by artificial tears combined chitosan, the control group were treated by artificial tears therapy. After treatment, patients were followed up for 1 to 6 months, observed and recorded the ocular function in two groups, as well as tear film breakup time (BUT), Schirmer I test (SIT), corneal fluorescein staining (FL) and visual function scores and other indicators, the effect of the two groups were compared and analyzed. Results: After treatment, tear film break-up time of the two groups increased significantly, and the observation group was higher, the difference was statistically significant (P<0.05); Schirmer I test values of two groups are significantly higher in the observation group than that in the control group, the difference was significant (P<0.05); and corneal fluorescein staining area of two groups reduced significantly, and the stained area of the observation group was less than that of the control group, the difference was statistically significant (P<0.05); at the same time visual function scores were significantly higher, and the visual in observation group score was higher than that in the control group, the difference was statistically significant (P<0.05). Conclusion: The use of chitosan in dry eye patients achieved good results, it contributes to the rehabilitation of patients with eye diseases, reduces the chance of sicker patients, so that patients can quickly return to normal life and reduce the psychological burden on the patient’s family, it is worthy of wider application.