一氧化氮对鼻咽癌CNE-2细胞增殖与凋亡的影响
| 作者: |
1张彩霞,
1刘阳云,
1刘庚勋,
1江文,
1曹杭,
1陈琼,
1常恩格,
1张纪帅
1 湖南师范大学第二附属医院解放军第163医院耳鼻咽喉头颈外科，长沙 410003 |
| 通讯: |
刘阳云
Email: lyyundr@aliyun.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.03.021 |
| 基金: | 湖南省研究生科研创新项目, CX2016B225 湖南省教育厅科学研究项目, 14C0697 |
摘要
目的：探讨外源性NO对CNE-2细胞增殖与凋亡的影响。方法：分别用不同浓度NO供体药物硝普钠(sodium nitroprusside，SNP)干预CNE-2细胞，观察细胞形态学变化、检测细胞的抑制率及细胞的凋亡和坏死率。结果：SNP以浓度依赖性方式抑制CNE-2细胞增殖，SNP 100，200，400，600，800，1 600，3 200 μmol/L各组细胞抑制率与药物浓度呈正相关；SNP以浓度依赖性方式促进CNE-2细胞凋亡，SNP(1 000 μmol/L)组细胞凋亡率较SNP(600 μmol/L)组显著增高(P<0.05)。结论：外源性NO能抑制CNE-2的增殖，促进CNE-2的凋亡，其抑制效应与NO浓度呈正相关。
关键词：
一氧化氮
硝普钠
鼻咽癌
凋亡
Effect of proliferation and apoptosis induced by nitric oxide on nasopharyngeal carcinoma CNE-2 cell
CorrespondingAuthor: LIU Yangyun Email: lyyundr@aliyun.com
DOI: 10.3978/j.issn.2095-6959.2017.03.021
Abstract
Objective: To investigate the effect of proliferation and apoptosis induced by exogenous NO on CNE-2 cells. Methods: CNE-2 cells were treated by different concentrations of sodium nitroprusside (SNP). The morphological changes of cells were observed, the viability and apoptosis of cells were examined. Results: SNP inhibited proliferation of CNE-2 cell in a concentration-dependent manner, CNE-2 cell were treated by SNP 100, 200, 400, 600, 800, 1 600, 3 200 μmol/L group respectively and the inhibition rate of the cells of each group was positively correlated with the concentration of SNP. SNP promote apoptosis of CNE-2 cell in a concentration-dependent manner; compared with SNP 600 μmol/L, flow cytometry analysis showed that the apoptosis rate of CNE-2 cells on the group treated by SNP 1 000 μmol/L increased significantly (P<0.05). Conclusion: Exogenous nitric oxide can inhibit CNE-2 proliferation and promote apoptosis. The inhibitory effect was positively correlated with the concentration of NO.