miRNA-451在膀胱癌细胞中的表达及对其生物学功能的影响
| 作者: |
1巢海潮,
2董志峰,
3张古月,
4胡梅凤,
3朱遵伟,
3包佑根,
3曾涛
1 江西省人民医院临床医学研究所，南昌 330006 2 南昌大学研究生院医学部2015级，南昌 330006 3 江西省人民医院泌尿外科，南昌 330006 4 江西省人民医院供应室，南昌 330006 |
| 通讯: |
曾涛
Email: Taozeng40709@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2016.09.021 |
| 基金: | 江西省自然科学基金面上项目, 20161BAB205278 江西省卫计委课题, 20161007 |
摘要
目的：检测微小RNA(microRNA，miRNA)在膀胱癌细胞中的表达及探讨其对膀胱癌细胞迁移、侵袭、黏附及增殖能力的影响。方法：采用实时荧光定量PCR(quantitative Real-time PCR，qPCR)检测miR-451在不同转移潜能膀胱癌细胞株T24、5637、J82中的表达；使用Lipo-2000脂质体将miR-451拟似物(miR-451 mimics)转染入5637细胞，qPCR验证其转染效率，采用划痕愈伤实验、Transwell实验、细胞黏附及MTT增殖实验分别检测细胞二维迁移、侵袭、黏附及增殖能力的变化。结果：miR-451在T24、5637及J82中的相对表达量分别为0.06±0.001、0.13±0.024、1(将J82细胞中其表达量标准化为1)，差异显著，具有统计学意义(P<0.01)；miR-451过表达后，5637细胞的迁移、侵袭、黏附能力及增殖率显著降低，具有统计学意义(P<0.05)。结论：miR-451在不同膀胱癌细胞中差异表达，其表达异常可影响癌细胞生物学功能，这为膀胱癌靶向分子治疗提供了新的切入点。
关键词：
miR-451
膀胱癌
表达差异
生物学功能
Expression of miRNA-451 and its impact on the biological behavior of bladder carcinoma cells
CorrespondingAuthor: ZENG Tao Email: Taozeng40709@sina.com
DOI: 10.3978/j.issn.2095-6959.2016.09.021
Abstract
Objective: To explore the expression of miR-451 and its effect on migration, invasion, adhesion and proliferation of bladder carcinoma cells. Methods: qPCR was used to measure the expressive level of miR-451 in bladder tumor cells (T24, 5637, J82) with different metastatic potentials. MiR-451 mimics were transfected into 5637 cells using LipofectamineTM 2000, the expression of miR-451 of transfected 5637 cells was measured with qPCR. The migration, invasion, adhesion and proliferation of 5637 was detected by wound healing assay, Transwell method, cell adhesion assay and MTT method, respectively. Results: The relative expression of miR-451 in T24, 5637, J82 was 0.06±0.001, 0.13±0.024, 1 respectively, P<0.01, with statistical significance. The migration, invasion, adhesion and proliferation of 5637 was decreased significantly after transfected with miR-451 mimics (P<0.05). Conclusion: Differential expressed miR-451 exist in different bladder cancer cells, the abnormal expression of miR-451 in 5637 cells may influence its biology function, and it is a new entry point for bladder cancer molecular targeting therapy.