146例套细胞淋巴瘤免疫组织化学结果分析
| 作者: |
1李晶,
2胡昌明,
1丁向东,
1冯晓冬,
2任玉霞,
1朱梅刚
1 广州金域医学检验中心有限公司实验诊断部组织病理科，广州 510330 2 广州金域医学检验中心有限公司实验诊断部分子病理科，广州 510330 |
| 通讯: |
朱梅刚
Email: zmg@fimmu.com |
| DOI: | 10.3978/j.issn.2095-6959.2016.06.003 |
摘要
目的：讨论套细胞淋巴瘤的免疫组织化学特征。方法：回顾性分析146例套细胞淋巴瘤的免疫组化结果，并用FISH方法检测1例Cyclin D1阴性病例是否存在t(11;14)易位。结果：套细胞淋巴瘤免疫组化阳性率为CD20：98.6%(144/146)；CD79a：100%(146/146)；CD5：88.4%(129/146)；CyclinD1：99.3%(145/146)；PAX-5：100%(122/122)；CD43：84%(79/94)；Ki67指数：5%~90%，中位数为20%。少部分病例异常表达CD10、Bcl6、CD23、CD56、CD3、CD45RO。FISH检测1例Cyclin D1阴性病例结果为检测到t(11;14)易位形成的IgH/CCND1融合基因。结论：套细胞淋巴瘤存在较为特征性的免疫组化表达模式，并存在异常表达现象。
关键词：
套细胞淋巴瘤
免疫组织化学
原位杂交
Immunohistochemical analysis of 146 cases of mantle cell lymphoma
CorrespondingAuthor: ZHU Meigang Email: zmg@fimmu.com
DOI: 10.3978/j.issn.2095-6959.2016.06.003
Abstract
Objective: To discuss the immunohistochemical features of mantle cell lymphoma (MCL). Methods: A retrospective analysis of immunohistochemical results of 146 cases of mantle cell lymphoma was performed, one cyclin D1 negative case was detected t(11;14) translocation with FISH. Results: The positive rate in 146 cases were CD20: 98.6%; CD79a: 100%; CD5: 88.4%; Cyclin D1: 99.3%; PAX-5: 100%; CD43: 84%; Ki67 index: 5%~90%. CD10, Bcl6, CD23, CD56, CD3 and CD45RO were positive in rare case. IgH/CCND1 fusion gene was substantiated in one cyclin D1 negative case. Conclusion: There is the characteristic expression pattern of immunohistochemistry in mantle cell lymphoma, and the abnormal expression existed.