MMP16在胰腺癌组织中的表达及其临床意义
| 作者: |
1何同梅,
1陈丽芳,
1何时,
2林凡,
1许春伟,
1陈燕坪
1 福建医科大学教学医院福建省肿瘤医院病理科，福州 350014 2 福建中医药大学附属人民医院肿瘤外科，福州 350000 |
| 通讯: |
陈燕坪
Email: kelf2006@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2016.10.026 |
| 基金: | 国家临床重点专科建设项目, 2013 福建省卫生厅青年创新课题, 2014-1-15 |
摘要
目的：检测胰腺癌组织中MMP16蛋白表达情况，探讨其在胰腺癌发生、发展中的作用、及其临床意义。方法：应用免疫印迹法检测8对胰腺癌/癌旁组织中MMP16蛋白的表达情况；应用免疫组化EnVision法检测49例胰腺癌组织中MMP16蛋白的表达情况，分析其与临床病理因素的关系。结果：免疫印迹法检测显示，胰腺癌组织MMP16蛋白的相对表达量均高于相应癌旁组织(8/8)；免疫组化检测显示，在胰腺癌组织中MMP16蛋白的表达率为85.7%(42/49)，而在癌旁正常组织中MMP16蛋白表达率为9.1%(3/33)；MMP16蛋白在癌组织中的表达率明显高于癌旁正常组织，差异有统计学意义(P<0.05)。MMP16表达与胰腺癌的组织分化程度、临床分期、淋巴结转移有关；该三因素的分组间均有统计学差异(P<0.05)。结论：MMP16在胰腺癌组织中呈高表达，因此可能在胰腺癌的发生、发展中起重要作用。
关键词：
胰腺癌
细胞外基质
基质金属蛋白酶
Expression and clinical significance of MMP16 in pancreatic carcinoma
CorrespondingAuthor: CHEN Yanping Email: kelf2006@126.com
DOI: 10.3978/j.issn.2095-6959.2016.10.026
Abstract
Objective: To detect the expression of MMP16 and to investigate its roles in the formation, development and clinical significance of human pancreatic carcinoma. Methods: Immunoblot was utilized to detect the expression of MMP16 in 8 paired pancreatic carcinoma and adjacent non-tumorous tissues; immunohistochemical staining was used to detect the expression of MMP16 in 49 pancreatic carcinoma tissues; the relationship between MMP16 and the clinical pathological factors was analyzed. Results: Immunoblot proved that the expression of MMP16 protein in pancreatic carcinoma was higher than that in matched adjacent non-tumorous tissues (8/8); immunohistochemical staining demonstrated that the expression rate of MMP16 in pancreatic carcinoma was 85.7%, significantly higher than that of normal group (9.1%), the difference was statistically significant (P<0.05). the positive expression of MMP16 was closely related to the degree of differentiation, clinical stages and lymph node metastasis There were significant differences among the groups (P<0.05). Conclusion: MMP16 is overexpressed in human pancreatic carcinoma, and may play an important role in the carcinogenesis and progress of pancreatic carcinoma.