AP-2α对肺腺癌A549细胞MnSOD表达的作用研究
| 作者: |
1杨欢,
1刘志光,
2周锐
1 湖南师范大学第一附属医院湖南省人民医院呼吸内科，长沙 410005 2 中南大学湘雅二医院呼吸内科，长沙 410011 |
| 通讯: |
周锐
Email: zhourui2355@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2016.07.006 |
| 基金: | 国家临床重点专科建设项目 |
摘要
目的：探讨转录因子激活蛋白-2α (activator protein-2 alpha，AP-2α)对锰超氧化物歧化酶(manganese superoxide dismutase，MnSOD)mRNA和蛋白表达的影响，探讨AP-2α下调MnSOD表达的可能的分子机制。方法：转染Ad-AP-2α和AP-2α siRNA进入A549细胞，上调和下调AP-2α后，采用实时定量RT-PCR及Western blot检测A549细胞内MnSOD的mRNA及蛋白质的表达水平。结果：1)Ad-AP-2α转染A549细胞后，MnSOD mRNA和蛋白质表达均下降；2)AP-2α siRNA转染A549细胞后，MnSOD mRNA及蛋白质表达均增加。结论：AP-2α对肺腺癌A549细胞MnSOD的mRNA和蛋白表达起下调作用。
关键词：
激活蛋白-2
锰超氧化物歧化酶
肺癌
Study on the role of AP-2α in the expression of MnSOD in the lung cancer A549 cells
CorrespondingAuthor: ZHOU Rui Email: zhourui2355@sina.com
DOI: 10.3978/j.issn.2095-6959.2016.07.006
Abstract
Objective: To investigate the effect of AP-2α on MnSOD gene/protein expression in lung cancer (A549) cells and its possible molecular mechanism of downregulation. Methods: A549 cells were transfected with adenovirus vector overexpressing AP-2α and AP-2α siRNA knockdowning AP-2α. The levels of AP-2α on MnSOD gene/protein expression were detected by RT-PCR and western blot. Results: 1) After transfected with Ad-AP-2α in A549 cells, the levels of MnSOD mRNA and protein expression both decreased; 2) the expression of mRNA and protein of MnSOD increased after AP-2α siRNA transfected in A549 cells. Conclusion: The results revealed that AP-2α can effectively down-regulate MnSOD gene/protein expression.