Objective: To determine the clinicopathologic features, immunohistochemistry, diagnosis and differential diagnosis of epithelial-myoepithelial tumor of breast. Methods: Clinical characteristics, pathological morphology and immunohistochemical staining in 4 cases of mammary epithelial-myoepithelial tumors were analyzed, and literature was reviewed. Results: The patients included 3 females and 1 male. The age of patients ranged from 27 to 63 years old (median=51 years). The size of the 4 tumors ranged from 1.5 to 3.0 cm in diameter (median=2.0 cm). Gross examination showed that the 4 tumors were gray-white and without true fibrous capsule. Microscopically, the feature of the tumor was double-layer tubular pattern, which composed of glandular epithelial and myoepithelial cells. The glandular epithelial cells were located in the inner layer of the tubular pattern, while the myoepithelial cells were located in the outer layer. Immunohistochemical results showed that the expression of CK8/18 and CK7 in glandular epithelial cells was positive, while the expression of p63, Calponin, CK5/6 in myoepithelial cells was positive. One case was diagnosed as adenomyoepithelioma, and 3 cases were diagnosed as malignant adenomyoepithelioma. Conclusion: Epithelial-myoepithelial tumor of breast is a rare neoplasm, which should be distinguished from intraductal papilloma, metaplastic carcinoma, and others.