Oxidative stress have an important role in the degeneration of dopaminergic neurons in Parkinson’s disease, and the origin and mechanism of ROS (reactive oxygen species, ROS), including the metabolism of dopamine and mitochondrial dysfunction. DJ-1 plays an antioxidant role in Parkinson’s disease and heart failure, which is involved in oxidative stress and protecting cells. DJ-1 is a tumor-associated protein, which is involved in different cell signaling pathways. DJ-1 can regulate the oxidative stress, which has a definite effect on the invasion and metastasis of tumor cells, thus it can be speculated that DJ-1 acts as an antioxidant which can affect the treatment and recurrence of tumor. It has now been confirmed that there is a close relationship between DJ-1 and cancer, but it is still unclear how it works and the mechanism of signaling pathways that alter cell survival. In this paper, we reviewed the progress of DJ-1 in the treatment of Parkinson’s disease, heart failure, tumor, anti-oxidative mechanism, and the target in tumor therapy.