MUC5B、Villin、P53在胆囊黏膜幽门腺化生、肠上皮化生及胆囊腺癌中的表达及意义
| 作者: |
1张东生,
1李岚,
1邱娜,
1张晶晶,
1卫陇,
1拓晓娟
1 兰州市第二人民医院病理科，兰州 730000 |
| 通讯: |
张东生
Email: bingli8365202@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2016.01.004 |
摘要
目的：通过观察MUC5B、Villin、P53蛋白在胆囊黏膜幽门腺化生、肠上皮化生及胆囊腺癌的表达，探讨胆囊黏膜两种化生与胆囊腺癌发生的关系。方法：收集2013年1月至2015年1月兰州市第二人民医院病理科诊断的胆囊黏膜幽门腺化生40例、肠上皮化生40例及胆囊腺癌40例，采用免疫组化方法检测MUC5B、Villin、P53在胆囊黏膜幽门腺化生、肠上皮化生及胆囊腺癌的表达。结果：MUC5B在胆囊黏膜幽门腺化生、肠上皮化生、胆囊腺癌的阳性表达率分别为95.00%(38/40)、75.00%(30/40)、27.50%(11/40)；Villin在三组中的阳性表达率分别为0.00%(0/40)、87.50%(35/40)、22.50%(9/40)；P53在三组中的阳性表达率分别为2.50%(1/40)、7.50%(3/40)、80.00%(32/40)。各组间比较MUC5B在幽门腺及肠上皮化生的阳性表达率明显高于胆囊腺癌，差异有统计学意义(χ2=42.754，P=0.001)；Villin在肠上皮化生的阳性表达率明显高于幽门腺化生及胆囊腺癌，差异有统计学意义(χ2=71.124，P=0.001)；P53在胆囊腺癌中的阳性表达率明显高于幽门腺及肠上皮化生，差异有统计学意义(χ2=71.667，P=0.001)。MUC5B、Villin在幽门腺化生、肠上皮化生及胆囊腺癌的阳性表达率递减；P53在幽门腺化生、肠上皮化生及胆囊腺癌的阳性表达率递增。结论：幽门腺及肠上皮化生可能参与了胆囊腺癌的发生。
关键词：
胆囊粘膜；幽门腺化生；肠上皮化生；胆囊腺癌；MUC5B； Villin； P53
Expression and significance of MUC5B, Villin, P53 in gallbladder mucosa pyloric gland metaplasia, intestinal metaplasia and gallbladder adenocarcinoma
CorrespondingAuthor: ZHANG Dongsheng Email: bingli8365202@163.com
DOI: 10.3978/j.issn.2095-6959.2016.01.004
Abstract
Objective: To investigate the relationship between two kinds of gallbladder mucosa metaplasia and gallbladder adenocarcinoma by observing the expression of MUC5B, Villin, P53 in gallbladder mucosa pyloric gland metaplasia, intestinal metaplasia and gallbladder adenocarcinoma. Methods: A total of 120 cases were collected in pathology department of the second people’s hospital of Lanzhou from January 2013 to January 2015, including 40 cases of gallbladder mucosa pyloric gland metaplasia, 40 cases of intestinal metaplasia and 40 cases of gallbladder adenocarcinoma. Immunohistochemical method was used to detect the expression of MUC5B, Villin, P53 in gallbladder mucosa pyloric gland metaplasia, intestinal metaplasia and gallbladder adenocarcinoma. Results: Positive expression rate of MUC5B was 95.00% (38/40), 75.00% (30/40), 27.50% (11/40) in gallbladder mucosa pyloric gland metaplasia, intestinal metaplasia, gallbladder adenocarcinoma respectively; positive expression rate of Villin was 0.00% (0/40), 87.50% (35/40), 22.50% (9/40) in three groups respectively; positive expression rate of P53 was 2.50% (1/40), 7.50% (3/40), 80.00% (32/40) in three groups respectively. Comparison between three groups, positive expression rate of MUC5B in pyloric gland and intestinal metaplasia was obviously higher than that of gallbladder adenocarcinoma, the difference was statistically significant (χ2=42.754, P=0.001); positive expression rate of Villin in the intestinal metaplasia was obviously higher than that of pyloric gland metaplasia and gallbladder adenocarcinoma, the difference was statistically significant (χ2=71.124, P=0.001); P53 positive expression rate in the gallbladder adenocarcinoma was obviously higher than that of pyloric gland and intestinal metaplasia, the difference was statistically significant (χ2=71.667, P=0.001). MUC5B, Villin positive expression rate decreased in turn among the three groups; P53 positive expression rate increased in turn among the three groups. Conclusion: The pyloric gland and intestinal metaplasia may participate in the occurrence of gallbladder carcinoma.