文章摘要

miR-136通过抑制PPP2R2A表达调控胃癌细胞SGC7901凋亡

作者: 1邢亚楠, 1邓鹏, 1徐惠绵
1 中国医科大学附属第一医院肿瘤外科，沈阳 110001
通讯: 邢亚楠 Email: cmu_yanan@163.com
徐惠绵 Email: cmu_directorHM@163.com
DOI: 10.3978/j.issn.2095-6959.2015.12.019
基金: 辽宁省科技计划项目, 2011225019

摘要

目的：检测miR-136对胃癌细胞SGC7901增殖和凋亡的影响及其机制。方法：分别利用MTT实验和Annexin V-PI实验检测转染miR-136 inhibitor的胃癌细胞SGC7901增殖和凋亡。利用western blot和双荧光素酶报告基因技术检测miR-136的靶基因。结果：抑制miR-136表达可以明显抑制SGC7901细胞增殖和诱导其凋亡。miR-136能够与PPP2R2A mRNA 3'-UTR区结合。结论：抑制miR-136表达能够促进PPP2R2A蛋白表达，进而引起胃癌细胞凋亡。
关键词： SGC7901细胞 miR-136 PPP2R2A 增殖 凋亡

miR-136 regulated apoptosis in gastric cell SGC7901 by suppressing PPP2R2A

Authors: 1XING Yanan, 1DENG Peng, 1XU Huimian
1 Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang 110001, China

CorrespondingAuthor: XING Yanan Email: cmu_yanan@163.com

DOI: 10.3978/j.issn.2095-6959.2015.12.019

Abstract

Objective: To detect the effects and mechanisms of miR-136 on gastric cancer cell SGC7901. Methods: The proliferation and the apoptosis of SGC7901 cells after transfected with miR-136 inhibitor were detected by MTT assay and Annexin V-PI assay, respectively. Western blot and dual luciferase reporter gene were used to detect the target gene of miR-136. Results: Inhibition of miR-136 could inhibit the proliferation and induce apoptosis in SGC7901 cells. miR-136 could combine with 3'-UTR of PPP2R2A mRNA. Conclusion: Inhibition of miR-136 could induce PPP2R2A expression and apoptosis in SGC7901 cells.

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