PPAR β/δ激动剂减轻小鼠心肌缺血/再灌注损伤
| 作者: |
1罗金文,
1陈仁伟,
1王敬华,
1刘平波
1 湖南省儿童医院心胸外科，长沙 410007 |
| 通讯: |
罗金文
Email: rockyman_luo@hotmail.com |
| DOI: | 10.3978/j.issn.2095-6959.2013.05.006 |
| 基金: | 湖南省卫生厅科研基金, 132013-105 |
摘要
目的：研究PPAR β/δ激动剂GW0742对小鼠心肌缺血/再灌注（ischemia-reperfusion，I/R）损伤
的影响。方法：将小鼠分成4组，分别为溶剂假手术组[二甲基亚砜(DMSO)， sham组]，激动剂假
手术组(GW0742 sham组)，溶剂手术组(DMSO I/R组)和激动剂手术组(GW0742 I/R组)。术后于小鼠
腹腔注射DMSO及GW0742。采用ELISA法检测各组血浆中的乳酸脱氢酶(lactate dehydrogenase，
LDH)，免疫印迹法检测各组核因子-κB(NF-κB) p65亚单位及细胞间黏附分子-1(ICAM-1)蛋白的表
达量。结果：GW0742 I/R组血浆中的LDH较DMSO I/R组减低 ( P<0.001)。GW0742 I/R组心肌中的NF-κBp65 和ICAM-1蛋白表达量较DMSO I/R组减低(分别为P<0.001和P<0.05)。结论：GW0742可以减轻小鼠心肌I/R损伤。PPAR β/δ是机体抗心肌I/R损伤的重要防御因子之一。
关键词：
过氧化物酶体增殖物激活型受体β/δ
GW0742
心肌
缺血/再灌注
的影响。方法：将小鼠分成4组，分别为溶剂假手术组[二甲基亚砜(DMSO)， sham组]，激动剂假
手术组(GW0742 sham组)，溶剂手术组(DMSO I/R组)和激动剂手术组(GW0742 I/R组)。术后于小鼠
腹腔注射DMSO及GW0742。采用ELISA法检测各组血浆中的乳酸脱氢酶(lactate dehydrogenase，
LDH)，免疫印迹法检测各组核因子-κB(NF-κB) p65亚单位及细胞间黏附分子-1(ICAM-1)蛋白的表
达量。结果：GW0742 I/R组血浆中的LDH较DMSO I/R组减低 ( P<0.001)。GW0742 I/R组心肌中的NF-κBp65 和ICAM-1蛋白表达量较DMSO I/R组减低(分别为P<0.001和P<0.05)。结论：GW0742可以减轻小鼠心肌I/R损伤。PPAR β/δ是机体抗心肌I/R损伤的重要防御因子之一。
PPAR β/δ agonist attenuates myocardial ischemiareperfusion injury in mice
DOI: 10.3978/j.issn.2095-6959.2013.05.006
Abstract
Objective: To determine the effect of peroxisome proliferator-activated receptor (PPAR) β/δ agonist GW0742 on myocardial ischemia-reperfusion (I/R) injury in mice. Methods: Mice were divided into 4 groups: A solventtreated sham group (DMSO sham group), a GW0742-treated sham group (GW0742 sham group), a solventoperated group (DMSO I/R group), and a GW0742-operated group (GW0742 I/R group). DMSO and GW0742 were intraperitoneally administered after operation. ELISA method was used to detect the plasma lactate dehydrogenase (LDH) and Western blot was used to detect the nuclear factor-κB (NF-κB) p65 and ICAM-1 protein expression in myocardium. Results: The concentration of LDH in serum was reduced significantly in the GW0742 I/R group compared with the DMSO I/R group (P<0.001). The protein expression of NF-κBp65 and ICAM-1 in myocardium reduced significantly in the GW0742 I/R group compared with the DMSO I/R group (P<0.001 and P<0.05, respectively). Conclusion: GW0742 can reduce myocardial I/R injury in mice by downregulation of ICAM-1 protein expression via activation of PPAR β/δ.