CREPT与CyclinD3在非小细胞肺癌中的表达及相关性研究
| 作者: |
1李维妙,
1夏靖华,
1王雪娇,
1王磊,
1文苗苗,
1孙盈,
1范亮波,
1邢昊,
1程庆书,
1张志培
1 第四军医大学唐都医院胸腔外科，西安 710038 |
| 通讯: |
程庆书
Email: chechest@fmmu.edu.cn 张志培 Email: zzpzyy@fmmu.edu.cn |
| DOI: | 10.3978/j.issn.2095-6959.2015.01.014 |
摘要
目的：检测CREPT(cell-cycle related and expression-elevated protein in tumor)与细胞周期蛋白 D(CyclinD3)在非小细胞肺癌(non-smal l cell lung cancer，NSCLC)中的表达，分析二者在促进 NSCLC发生发展过程中的作用。方法：免疫组织化学法检测CREPT、CyclinD3在271例NSCLC 及相应正常组织中的表达情况，统计学分析二者间的相关性及其在临床病理因素中的差异性。 结果：CREPT和Cycl inD3定位于细胞核与细胞质中，在NSCLC组织中高表达，癌组织与正常 组织中阳性表达率差异性极显著(P<0.001)。CREPT、CyclinD3在腺癌、鳞癌间表达无统计学 差异(P=0.638，P=0.503)；在总NSCLC、腺癌、鳞癌的病理分级间，表达均具有极显著性差 异(P<0.001)；在总NSCLC组织TNM分期、淋巴结是否转移间的表达差异性显著(P=0.025， P=0.001；P=0.003，P=0.026)；在总NSCLC、腺癌、鳞癌中，CREPT和Cyc linD3表达秩相关 系数r分别是0.458、0.393、0.468；P<0.001，二者具有正相关性。结论：CREPT和CyclinD3是 NSCLC组织与相应正常组织表达的差异蛋白，二者的过表达可能在NSCLC的发生发展过程中起 作用。
关键词：
非小细胞肺癌
CREPT
CyclinD3
Expression and correlation of CREPT and CyclinD3 in non-small cell lung cancer
CorrespondingAuthor: CHENG Qingshu Email: chechest@fmmu.edu.cn
DOI: 10.3978/j.issn.2095-6959.2015.01.014
Abstract
Objective: To investigate the expression of CREPT and CyclinD3 in NSCLC, and the role of them in NSCLC occurrence and development process. Methods: The expression of CREPT and CyclinD3 in 271 pairs of NSCLC and corresponding normal tissues were detected by immunohistochemical method. The correlation between CREPT and CyclinD3 expression as well as the difference in clinico-pathological parameters were analyzed by statistical method. Results: Positive staining of CREPT and CyclinD3 were in cytoplasm and nucleus. The positive expression rates of the CREPT and CyclinD3 protein in NSCLC was significantly higher than those in the corresponding normal tissue (all P<0.001). The expression of CREPT and CyclinD3 were no significant differences in among groups of lung adenocarcinoma and squamous cell lung cancer (P=0.638; P=0.503), but there were extremely significant difference in pathological grading groups of NSCLC, adenocarcinoma, squamous cell carcinoma (all P<0.001). There were significant difference in the CREPT and CyclinD3 expression among various groups of clinical stages and lymph node metastasis or not (P=0.025, P=0.001; P=0.003, P=0.026). The protein expression level of CREPT was positively related with that of CyclinD3 in NSCLC, adenocarcinoma, squamous cell carcinoma(r=0.458, r=0.393, r=0.468; all P<0.001). Conclusion: Our data suggest that CREPT and CyclinD3 were differentially expressed proteins in NSCLC tissues and corresponding normal tissues and those over-expressed proteins being crucial for NSCLC occurrence and development