Background: This study was attempted to evaluate the pathogen characteristic of seven respiratory viruses found in children with respiratory tract infections in Wuxi of Jiangsu Province. Methods: In this work, a total of 340 nasal and throat swabs were collected from June 2012 to May 2014 in Wuxi NO.2 People’s Hospital. Seven respiratory viruses including influenza virus A (FA), influenza virus B (FB), parainfluenza virus I (PVI), parainfluenza virus I (PVII), parainfluenza virus III (PVIII), adenovirus (ADV) and respiratory syncytial virus (RSV) were detected using D3 Ultra TM DFA Respiratory Virus Screening & ID Kit. Gender disparities, age distribution, seasonal distribution were analyzed in our study. Results: Viral pathogens were detected in 34.12% (116/340) of patient samples, the top three common viruses identified was RSV (16.18%; 55/340), followed by FB (5.29%; 18/340) and FA (5.00%; 17/340). There was no statistical difference of the detection rates between male (36.32%; 73/201) and female (31.65%; 44/139). Most viral infection occurred in youngest patients of no more than 1 year (48.48%; 32/66). It varied by season, being more prevalent in winter (42.72%; 44/103). Conclusion: RSV was the most common detected respiratory virus in Wuxi. Infant 0-1 years of age should be taken as a key phase in the prevention of respiratory virus. Respiratory infection of children usually breaks out in winter. This study adds to our knowledge of the epidemiology characteristics of the seven common respiratory viruses among children in Wuxi.