Objective: To investigate the prognostic factors of urosepsis after surgery with urologic endoscopy and to improve the prognosis of urosepsis. Methods: A retrospective analysis of clinical data of 57 patients was carried who occurred urosepsis after surgery with urologic endoscopy. These cases were divided into low-risk group and high risk group and compared the difference of clinical data and laboratory results. Results: Of 57 cases of urosepsis, 24 patients were divided into high-risk group and 33 cases into low risk group. There were significant differences in the ratio of male/female, urinary tract infection and diabetes mellitus between high-risk group and the low-risk group. In terms of operation, ureter surgery in high-risk group was higher than low risk level, but percutaneous nephrolithotripsy was significantly lower than the low-risk group, and surgical time of highrisk group was significantly longer than that of low-risk group. Postoperative blood leukocytes, neutrophils, C-reactive protein, multiple organ dysfunction, blood culture positive ratio in the high-risk group is higher than the low-risk group, and the time interval from surgery to uroseptic symptoms occurring (interval time of surgery-infection), fibrinogen, platelets, hemoglobin in high risk group were lower than low risk group. Conclusion: Male, urinary tract infection, diabetes mellitus, ureteroscopy and longer surgical time are the factors of poor prognosis of urosepsis; significantly higher blood neutrophils after surgery, elevated c-reactive protein, multiple organ dysfunction, positive blood culture, shorter interval time of surgical-infection and lower platelets, lower hemoglobin, lower fibrinogen are the poor prognostic indicators.