不同检测方法检测梅毒螺旋抗体的效果比较分析
| 作者: |
1吕志军
1 张家港市红十字血站检验科，江苏 张家港 215600 |
| 通讯: |
吕志军
Email: 34265174@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2014.06.013 |
摘要
目的：比较TP-CL IA、TP-E L I S A 和TPPA三种检测方法检测梅毒螺旋抗体的效果差异。 方法：2012年1月至2014年1月期间我院门诊共收集到窗口期疑似病人180例分别用化学发光法 (TP-Chemiluminescence immunoasy，TP-CLIA)、酶联免疫吸附试验(TP-enzyme-linked immunosorbent assay，TP-ELISA)和梅毒螺旋体明胶颗粒凝聚试验(TPPA)三种方法检测血清样本，比较三种检测 方法的敏感性、特异性。结果：TP-CLIA法灵敏度为99.4%，特异性为100.0%；TP-ELISA法灵敏度 97.8%，特异性95.7%；TPPA法灵敏度97.2%，特异性97.5%。结论：TP-CLlA法作为新开发的梅毒抗 体检测方法，可作为梅毒的确诊实验，TP-ELISA法和TPPA法可作为TP-CLlA法的补充。
关键词：
梅毒螺旋体
化学发光法
酶联免疫吸附试验
梅毒螺旋体明胶颗粒凝聚试验
The effect of different testing methods syphilis antibody of spiral comparative analysis
CorrespondingAuthor: LV Zhijun Email: 34265174@qq.com
DOI: 10.3978/j.issn.2095-6959.2014.06.013
Abstract
Objective: To compare the test performance of syphilis antibody in the specimen serum with the TP-CLIA, TP-ELISA and TPPA methods. Methods: A total of 180 cases of suspected patients during the window period were collected from January 2012 to January 2014. The serum samples were detected using the method of chemiluminescence (TP-CLIA), enzyme-linked immunosorbent assay (TP-ELISA) and treponema pallidum gelatin particle coagulation test (TPPA), respectively. The data of sensitivity and specificity of three methods were collected and compared. Results: The data of the sensitivity of TP-CLIA method was 99.4%, the data of the specificity was 100.0%; the data of the sensitivity of TP-ELISA method was 97.8%, the data of the specificity was 97.8%; the data of the sensitivity of the TPPA method was 97.2%, the data of the specificity was 97.5%. Conclusion: TP-CLlA method as a new development of syphilis antibody can be used for diagnosis of syphilis experiments. TP-ELISA and TPPA method can be used to supplement the TP-CLlA method.