CYP1B1及MDR1在两种人胃癌细胞中的差异表达
| 作者: |
1张建新,
2鲍柏军
1 江苏省如东县人民医院消化内科，江苏 如东 226400 2 南通大学附属医院消化内科，江苏 南通 226001 |
| 通讯: |
鲍柏军
Email: bamboo@medmail.com.cn |
| DOI: | 10.3978/j.issn.2095-6959.2014.06.011 |
摘要
目的：比较细胞色素P4501B1(CYP1B1)及多药耐药基因(MDR1)在人胃癌细胞SGC7901和 SGC7901/VCR细胞中的差异表达。方法：采用实时荧光定量PCR法检测CYP1B1及MDR1在二种人 胃癌细胞中的表达情况。结果：CYP1B1及MDR1在SGC7901/VCR细胞中的表达量(0.726±0.074及 0.545±0.068)明显高于SGC7901细胞(0.391±0.045及0.321±0.084，P<0.05)，且两者在SGC7901/VCR 细胞中的表达呈明显正相关(r=0.7859，P=0.0120)。结论：CYP1B1及MDR1可能共同参与了胃癌细 胞对长春新碱(vincristine，VCR)的耐药。
关键词：
胃癌
细胞色素4501B1
多药耐药基因
The differential expression of CYP1B1 and MDR1 in two human gastric cancer cells
CorrespondingAuthor: BAO Baijun Email: bamboo@medmail.com.cn
DOI: 10.3978/j.issn.2095-6959.2014.06.011
Abstract
Objective: To study the differential expression of CYP1B1 and MDR1 in human gastric cancer SGC7901 cells and SGC7901/VCR cells. Methods: The real-time fluorescent quantitation polymerase chain reaction (QT-PCR) was used to detect the differential expression of CYP1B1 and MDR1. Results: The expressions of CYP1B1 and MDR1 in SGC7901/VCR cells (0.726±0.074 and 0.545±0.068) were higher than that in SGC7901 cells (0.391±0.045 and 0.321±0.084, P<0.05), and there was a significant positive correlation expression between CYP1B1 and MDR1 in SGC7901/VCR cells (r=0.7859, P=0.0120). Conclusion: CYP1B1 and MDR1 may be involved in the gastric cancer cells to resist vincristine (VCR).