人源化小鼠异位肺癌动物模型的构建
| 作者: |
1刘君,
2刘翔,
1崔飞,
1黄丽燕,
1唐海玲,
3李慧灵,
1何建行
1 广州医科大学第一附属医院胸外科，广州市呼吸疾病研究所，广州 510515 2 南华大学附属第二医院胸心外科，湖南 衡阳 421001 3 中国人民解放军总医院海南分院呼吸内科，海南 三亚 572013) |
| 通讯: |
刘君
Email: liujun9707@sina.com 李慧灵 Email: lihuiling1975@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2014.04.009 |
摘要
目的：探讨一种可用于研究肺癌免疫的人源化小鼠动物模型。方法：采用人外周血单个核细胞 输注非肥胖糖尿病/严重联合免疫缺陷小鼠体内建立人源化小鼠，在此小鼠模型基础上再构建异 位肺癌荷瘤模型。结果：10只人源化小鼠异位荷瘤模型全部构建成功，免疫重构的小鼠外周血 及脾脏中均可检测到大量人CD3+T、 CD4+T、CD8+T细胞。人源化荷瘤小鼠肿瘤组织内可发现人 CD3+T、 CD4+T、CD8+T淋巴细胞浸润。结论：该模型为我们了解肺癌发生发展与免疫系统的关 系，也为研究免疫治疗干预措施等提供了有价值的工具。
关键词：
人源化；肺癌；动物模型；肿瘤浸润淋巴细胞
Establishment of humanized lung cancer heterotopic mouse model
CorrespondingAuthor: LIU Jun Email: liujun9707@sina.com
DOI: 10.3978/j.issn.2095-6959.2014.04.009
Abstract
Objective: To explore a lung humanized mouse model which can be used cancer immunization. Method: Human peripheral blood mononuclear cells through the were injected intraperitoneally into Non-obese diabetic/severe combined immunodeficient (NOD/SCID) chimeric mice to constructed humanized model. The re-construction of a mouse lung cancer heterotopic model on basis of the humanized model. Results: 100% success rate of 10 lung cancer heterotopic humanized mice. Human CD3+T, CD4+T, CD8+T cells were highly expressed in mouse spleen after immune reconstruction by immunohistochemical detection. Human CD3+T, CD4+T, CD8+T cells were found infiltrating in the tumor tissue of tumor-bearing humanized mice. Conclusion: The model provide a valuable tool for our understanding of the relationship between the development of lung cancer but also for the study of immunotherapy interventions.