文章摘要

血浆载脂蛋白A5 水平与HDL 亚类分布的关系

作者: 1韩瑛, 2陈志军, 3唐伟军, 4龙石银, 5卢新华, 6邓华菲, 3王岐本, 4田英
1 1. 湘南学院基础医学部机能学实验室，湖南 郴州 423000；2. 湘南学院南岭药用资源研究所， 湖南 郴州 423000
2 3.郴州市第一人民医院病理诊断中心， 湖南 郴州 423000
3 湘南学院南岭药用资源研究所， 湖南 郴州 423000
4 南华大学生物化学与分子生物学教研室，湖南 衡阳 421001
5 湘南学院基础医学部机能学实验室，湖南 郴州 423000；湘南学院南岭药用资源研究所， 湖南 郴州 423000
6 湘南学院基础医学部机能学实验室，湖南 郴州 423000
通讯: 田英 Email: uscty@163.com
基金: 湖南省教育厅科研基金重点项目(12A127)；湖南省卫生厅科研基金(B2012-74)；湘南学院科研基金(2013YJ46)

摘要

目的：探讨载脂蛋白A5(apolipoprotein A5, apoA5)水平对HDL亚类分布的影响。方法：采用酶联免疫吸附法(enzyme-linked immunosorbent assay，ELISA)和双向电泳免疫印迹检测法分别检测310例受试者的血浆apoA5浓度和HDL亚类相对含量。受试者按apoA5浓度均值加或减去一个标准差作为分割点，分为高apoA5组(apoA5≥286.83 mg/L)，中apoA5组(139.99 mg/L≤apoA5<286.83 mg/L)和低apoA5组(apoA5<139.99 mg/L)。结果：与高apoA5组相比，apoA5低浓度组preβ1-HDL和HDL3a含量显著升高(P<0.01)，HDL2a和HDL2b显著降低(P<0.01)。结论：随着血浆apoA5浓度降低，HDL亚类有变小的趋势，apoA5浓度降低可能阻碍了HDL亚类的成熟。
关键词： HDL亚类 载脂蛋白A5 双向电泳-免疫印迹检测法

Relationship between apoA5 level and HDL subclasses

Authors: 1HAN Ying, 2CHEN Zhijun, 3TANG Weijun, 4LONG Shiyin, 5LU Xinhua, 6DENG Huafei, 3WANG Qiben, 4TIAN Ying
1 1. Functional Laboratory, Department of Basic Medicine, University of Xiangnan, Chenzhou Hunan 423000; 2. Joint Institute for Medicinal Resources Nanling, University of Xiangnan, Chenzhou Hunan 423000;
2 Pathological Diagnosis Center, First People's Hospital of Chenzhou, Chenzhou Hunan 423000
3 Joint Institute for Medicinal Resources Nanling, University of Xiangnan, Chenzhou Hunan 423000
4 Department of Biochemistry and Molecular Biology, University of South China, Hengyang Hunan 421001, China
5 Functional Laboratory, Department of Basic Medicine, University of Xiangnan, Chenzhou Hunan 423000; Joint Institute for Medicinal Resources Nanling, University of Xiangnan, Chenzhou Hunan 423000
6 Functional Laboratory, Department of Basic Medicine, University of Xiangnan, Chenzhou Hunan 423000

CorrespondingAuthor: TIAN Ying Email: uscty@163.com

Abstract

Objective: To explore the relation between the apolipoprotein A5 (apoA5) levels and subclasses of high density lipoprotein (HDL). Methods: The plasma level of apoA5 was detected by enzyme-linked immunosorbent assay (ELISA), and the HDL subclasses were measured with two-dimensional electrophoresis immunoblot in 310 subjects. According to the apoA5 level, the subjects were divided into three groups: a high level of apoA5 (apoA5 ≥ 286.83 mg/L) group, a medium level of apoA5 (139.99 ≤ apoA5 < 286.83 mg/L) group and a low level of apoA5 (apoA5 < 139.99 mg/L) group. Results: Compared with the high level of apoA5 group, preβ1-HDL and HDL3a were significantly increased (P<0.01), while HDL2a and HDL2b were significantly decreased (P<0.01) in the low level of apoA5 group. Conclusion: With the decreased plasma level of apoA5, the particle size of HDL shows a shift toward smaller size, and the decreased apoA5 may suppress the mature metabolism of HDL.

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