文章摘要

条件性剔除鼠在神经元发育过程中的应用

作者: 1刘长红, 1武明花, 2李桂源
1 中南大学肿瘤研究所，卫生部癌变原理重点实验室，教育部癌变与侵袭原理重点实验室； 湖南省颅底外科与神经肿瘤临床医疗技术研究中心，长沙 410078
2 中南大学肿瘤研究所，卫生部癌变原理重点实验室，教育部癌变与侵袭原理重点实验室
通讯: 武明花 Email: wuminghua554@aliyum.com
DOI: 10.3978/j.issn.2095-6959.2014.02.011
基金: 国家自然科学基金, 81272297

摘要

条件性基因敲除技术是最近发展起来的新技术，常用的敲除系统有环化重组酶/环化重组酶识别的特异性DNA序列系统、成髓细胞瘤基因转录因子重组酶/铁蛋白位点系统、四环素诱导系统、锌指核酸酶技术、转录激活因子样效应因子核酸酶技术、短回文重复序列/短回文重复序列相关基因系统。该技术能够定向地修饰和改造基因，能够在时间和空间上控制基因敲除，如今已发展成为研究脊椎动物成体中基因功能的重要手段，尤其是该技术在小鼠中的应用更是取得了显著的成果。
关键词： 条件性剔除技术；常用系统；功能研究；展望

Application of conditional knockout mice in neuron development

Authors: 1LIU Changhong, 1WU Minghua, 2LI Guiyuan
1 Cancer Research Institute, Central South University; Key Laboratory of Carcinogenesis of Ministry of Health; Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education; Center for Skull Base Surgery and Neuro-oncology of Hunan Province, Changsha 410078, China
2 Cancer Research Institute, Central South University; Key Laboratory of Carcinogenesis of Ministry of Health; Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education;

CorrespondingAuthor: WU Minghua Email: wuminghua554@aliyum.com

DOI: 10.3978/j.issn.2095-6959.2014.02.011

Abstract

Conditional knockout technology is a newly developed technology, which adopted common knockout systems, such as Cre-LoxP system, myeloblastosis oncegene transcription factor/ferritin system, tetracycline-dependent regulatory system, zinc finger nuclease, transcription activator-like effector nucleases and clustered rugularly interspaced short palindromic repeat sequences (CRISPR)/CRISPR-associated gene system. This technology can modify the target genes and delete them under the control in space and time. The conditional knockout technology has become an important strategy to study gene function in adult vertebrates. In particular, the technology application in mice has made significant progress.

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