心房颤动患者心房组织中差异表达miRNA的初步研究
| 作者: |
1李闺琴,
2张磊善,
3杨晓燕,
4唐惠芳,
3雷小勇
1 南华大学附属南华医院临床药学室，湖南 衡阳 421001 2 石门县人民医院临床药学室，湖南 石门 415300 3 南华大学药学与生物科学学院，湖南 衡阳 421001 4 南华大学附属第一医院医务部，湖南 衡阳 421001 |
| 通讯: |
雷小勇
Email: Leixiaoyong@aliyun.com |
| DOI: | 10.3978/j.issn.2095-6959.2014.01.004 |
| 基金: | 国家自然科学基金；衡阳市科技厅科研项目, 30900625/H0205;2012KS13 |
摘要
目的：应用基因芯片技术研究心房颤动(房颤)患者心房组织中miRNA的表达谱，分析差异表达的miRNA，为进一步研究miRNA在房颤发生、发展中的作用奠定基础。方法：采用miRNA基因芯片技术检测房颤患者和非房颤患者心房组织样本中miRNA的表达水平；采用实时定量PCR(quantitative real-time PCR，qRT-PCR)对部分差异表达的miRNA进行验证。结果：MiRNA基因芯片分析结果显示，与非房颤组织相比，房颤组织中差异表达的miRNA共有26个，其中16个miRNA表达上调，10个miRNA表达下调。qRT-PCR验证结果与芯片结果相一致。结论：MiRNA在房颤患者心房组织中存在差异性表达。
关键词：
基因芯片；心房颤动；miRNA；差异表达
Preliminary study on differentially expressed microRNA in atrial tissues from patients with atrial fibrillation
CorrespondingAuthor: LEI Xiaoyong Email: Leixiaoyong@aliyun.com
DOI: 10.3978/j.issn.2095-6959.2014.01.004
Abstract
Objective: To analyze the differentially expressed microRNAs (miRNA) in atrial tissues from patients with atrial fibrillation by gene chip technology and to lay a foundation for studing its role in the development of atrial fibrillation. Methods: The miRNA gene chip was used to detect the miRNA expression level in patients with atrial fibrillation and without atrial fibrillation. Quantitative real-time PCR (qRT-PCR) was used to verify the differential expression of microRNAs.Results: The results of miRNA gene chip showed that there were 26 differentially expressed miRNAs in the atrial fibrillation group compared with the non-atrial fibrillation group. Among them, the expression levels of 16 miRNAs were up-regulated while 10 of them were down-regulated. The result of miRNA gene chip was in consistent with the result of qRT-PCR. Conclusion: The differential expression of miRNAs exists in the atrial tissues of patients with atrial fibrillation.