采用抗体芯片筛选鼻咽癌放射治疗抵抗相关的炎性因子
| 作者: |
1李娇阳,
1瞿家权,
1易红,
1易红梅,
1肖志强
1 中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室，长沙 410008 |
| 通讯: |
肖志强
Email: zqxiao2001@hotmail.com |
| DOI: | 10.3978/j.issn.2095-6959.2014.01.002 |
| 基金: | 国家自然科学基金, 81230053，81172559 |
摘要
目的：采用抗体芯片筛选鼻咽癌放射治疗(放疗)抵抗相关的炎性因子。方法：以放疗抵抗鼻咽癌CNE2-IR细胞与放疗敏感鼻咽癌CNE2细胞的细胞蛋白质及其细胞培养上清为样本，采用抗体芯片(AAH-INF-3)比较炎性因子的表达差异，采用免疫组织化学方法检测差异炎性因子IL-8在放疗抵抗与敏感鼻咽癌组织(每组30例)中的表达。结果：炎性因子IL-8、粒细胞-巨噬细胞集落刺激(granulocyte-macrophage colony stimulating factor，GM-CSF)和细胞间黏附分子-1(intercellular adhesion molecule 1，ICAM-1)在CNE2-IR细胞中的表达水平高于CNE2细胞，CNE2-IR细胞分泌的IL-8和GM-CSF水平也高于CNE2细胞，而CNE2-IR细胞分泌的基质金属蛋白酶-2(matrixmetalloproteinase 2，TIMP-2)水平低于CNE2细胞； IL-8在放疗抵抗鼻咽癌组织中的表达明显高于放疗敏感鼻咽癌组织。结论：IL-8，GM-CSF，ICAM-1和TIMP-2表达或分泌异常可能在鼻咽癌放疗抵抗中具有重要作用，为进一步研究鼻咽癌放疗抵抗的分子机制提供了新线索。
关键词：
鼻咽癌；放射治疗抵抗；抗体芯片；炎性因子
Identification of inflammation factors related to radioresistance of nasopharyngeal carcinoma by an antibody array
CorrespondingAuthor: XIAO Zhiqiang Email: zqxiao2001@hotmail.com
DOI: 10.3978/j.issn.2095-6959.2014.01.002
Abstract
Objective: To screen inflammation factors related to radioresistance of nasopharyngeal carcinoma (NPC).Methods: Antibody array of human inflammation factors (AAH-INF-3) were used to compare the expression differences between radioresistant NPC CNE2-IR and radiosensitive NPC CNE2 cells. Immunohistochemistry was performed to detect the expression levels of differential inflammation factor IL-8 in the radioresistant and radiosensitive NPC tissues (30 cases for each group). Results: The expression levels of IL-8, granulocyte-macrophage colony stimulating factor (GM-CSF) and intercellular adhesion molecule 1 (ICAM-1) in CNE2-IR cells were significantly higher than those in CNE2 cells, and CNE2-IR cells secreted more IL-8 and GM-CSF than CNE2 cells while secreted lesser matrixmetalloproteinase 2 (TIMP-2) than that in CNE2 cells. Immunohistochemistry showed that the expression levels of IL-8 were significantly higher in the radioresistant NPC tissues than those in the radiosensitive NPC tissues. Conclusion: Abnormal expression or secretion of IL-8, GM-CSF, ICAM-1, and TIMP-2 may play an important role in NPC radioresistance, which may provide a novel clue for further investigating the molecular mechanism of NPC radioresistance.