Objective: This study aim to investigated drug resistance to Acinetobacter baumannii, analyze the relationship between antibiotic use and resistance rates. Methods: The drug resistance of 665 strains of Acinetobacter Bauman was analyzed retrospectively, the drug consumption was analyzed by using frequency analysis and the relationship between antibiotic use and resistance rates were analyzed by SPSS. Results: Acinetobacter Bauman has a high resistance to a variety of commonly used antimicrobial agents, and there is a tendency of wider range of drug resistance. The resistance rates of cephalosporins and carbapenem drug increase significantly. There were Pearson significant relationship between use of three antibiotics and resistance in Acinetobacter Baumannii to this drug, Piperacillin/tazobactam (r=0.98, P<0.01), Gentamicin (r=0.87, P<0.01), Czidime (r=0.75, P<0.05), Cefoxitin (r=-0.74, P<0.05). Cefoperazone/sulbactam usage was also negatively correlated with aminoglycoside use frequency (r=-0.84, P<0.05), the drug resistance rate of Amikacin was positively correlated with the frequency of Cephalothin drug usage (r=0.98, P<0.01). Conclusion: The drug resistance to common antibiotics by Bauman is more serious, and there is a certain correlation between the use of antibacterial drugs and the rate of bacterial resistance.