卡维地洛对大鼠颈动脉损伤后血管重塑的影响
| 作者: |
1袁向珍,
1刘敏,
1曹中朝,
1包秋红
1 内蒙古医科大学附属医院保健中心，呼和浩特 010050 |
| 通讯: |
刘敏
Email: liuminzw@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2015.01.020 |
| 基金: | 内蒙古自治区自然科学基金项目, 2014MS08113 |
摘要
目的：探讨卡维地洛(carvedilol，CAR)对大鼠颈动脉损伤后血管重塑的影响。方法：雄性Wistar 大鼠90只，随机分为假手术组、损伤组和CAR组，后两组行颈动脉球囊损伤术。三组均于术后 1、3、7、14、28 天处死大鼠。光镜下观察血管损伤后内膜增生情况，用免疫组化和RT-PCR法 检测MMP-2、MMP-9和TIMP-1在各组术后不同时间点的表达情况。结果：与损伤组比较，术 后14dCAR组内膜面积、内膜与中膜面积比值显著减少，管腔面积显著扩大(P<0.05)；与损伤 组比较，CAR组术后3-14 d MMP-2、MMP-9表达显著减少(P<0.05)，而TIMP-1表达无显著变化 (P>0.05)。结论：卡维地洛有效抑制大鼠颈动脉损伤后MMP-2和MMP-9表达，改善了细胞外基质 的合成与降解平衡，抑制血管重塑，减轻再狭窄。
关键词：
卡维地洛
血管重塑
基质金属蛋白酶
Effect of carvedilol on vascular remodeling in carotid artery-injured rats
CorrespondingAuthor: LIU Min Email: liuminzw@126.com
DOI: 10.3978/j.issn.2095-6959.2015.01.020
Abstract
Objective: To explore the effect of carvedilol (CAR) on vascular remodeling in carotid artery-injured rats. Methods: Ninety Wistar rats were randomly divided into sham-operated group, injury group and CAR group, and the model of carotid balloon injury were established in the latter two groups. The rats were killed on the 1st、3rd、7th、 14th、28th day after injury. The intimal proliferation was observed by optical microscope. The expression of MMP-2, MMP-9 and TIMP-1 at different time points was detected by the method of immunohistochemistry and RT-PCR. Results: The intimal area (IA) and ratio of intimal and medial area (IA/MA)in CAR group were significantly lower than those in injury group, while lumen area was higher than its in injury group (P<0.05). Compared to injury group, the expression of MMP-2 and MMP-9 significantly decreased in CAR group on the 3rd to 14th day after injury (P<0.05), while the expression of TIMP-1 had no difference between two groups. Conclusion: CAR could inhibit vascular remodeling and reduce restenosis, the mechanism of which may inhibit the expression of MMP-2 and MMP-9 in carotid artery-injured rats, improve balance of synthesis and degradation of extracellular matrix.