Objective: To investigate the value of combined detection of serum interleukin-17 (IL-17) and IL-18 in the evaluation of sepsis complicated with acute kidney injury (AKI). Methods: One hundred and twenty patients with sepsis admitted to Dongfang People’s Hospital from November 2019 to November 2021 were selected and divided into an AKI group (n=42) and a non-AKI group (n=78) according to whether they had AKI. At the same time, patients with AKI were divided into stage I group, stage II group, and stage III group according to the staging standard of the Kidney Disease: Improving Global Outcomes (KDIGO). The expression levels of serum IL-17 and IL-18 in different groups were compared, and the correlation between the clinical staging of patients with sepsis complicated with AKI and serum IL-17 and IL-18 was analyzed. The clinical value of the combined diagnosis of sepsis complicated with AKI was also analyzed. Results: The serum levels of IL-17 and IL-18 in the AKI group were significantly higher than those in the non-AKI group (both P<0.05). Comparison of serum IL-17 and IL-18 levels in different AKI staging groups showed that stage III group > stage II group > stage I group (P<0.05). Spearman rank correlation analysis showed that AKI staging was significantly positively correlated with serum IL-17 and IL-18 levels (P<0.05). The area under the receiver operating characteristic (ROC) curve (AUC) of serum IL-17, IL-18, IL-17 combined with IL-18 in the diagnosis of sepsis complicated with AKI was 0.826, 0.677, and 0.904, respectively. The value of IL-17 combined with IL-18 in the evaluation of sepsis complicated with AKI was higher than that of IL-17 and IL-18 alone (P<0.05). Conclusion: Serum IL-17 and IL-18 levels in patients with sepsis complicated with AKI are significantly elevated, and the combined detection of the two has high value for the early diagnosis of sepsis complicated with AKI.