101例胃黏膜幽门螺杆菌感染耐药基因突变分析
作者: |
1娄丽萍,
2张文迪
1 华中科技大学同济医学院附属同济医院病理研究所,武汉 430030 2 华中科技大学同济医学院附属同济医院儿科,武汉 430030 |
通讯: |
张文迪
Email: wdzhang@outlook.com |
DOI: | 10.3978/j.issn.2095-6959.2022.09.005 |
摘要
Gene mutation and drug resistance analysis for 101 cases of gastric Helicobacter pylori infection
CorrespondingAuthor: ZHANG Wendi Email: wdzhang@outlook.com
DOI: 10.3978/j.issn.2095-6959.2022.09.005
Abstract
Objective: To compare the infection rate of Helicobacter pylori (Hp) in gastric mucosal biopsy samples by fluorescence quantitative PCR and immunohistochemistry, and to evaluate the feasibility of fluorescence quantitative PCR in detecting the resistance of Hp to clarithromycin and levofloxacin. Methods: A total of 101 cases of Hp infection in gastric mucosa were detected by fluorescence quantitative PCR and immunohistochemistry, and the infection rates of the 2 methods were compared. Then, 23S rRNA and gyrA gene mutations in Hp positive samples were detected by fluorescence quantitative PCR, and the mutation types of 23S rRNA gene and gyrA gene were analyzed by Sanger sequencing. Results: Among 101 gastric mucosa samples, 70 cases (69.31%) were Hp positive and 31 cases (30.69%) were Hp negative by PCR; 68 cases (67.33%) were Hp positive and 33 cases (32.67%) were Hp negative by immunohistochemistry. There was no significant difference in the positive rate of Hp between the 2 methods (P>0.05); Among 70 Hp positive samples detected by fluorescence quantitative PCR, 30 cases were clarithromycin resistant (42.25%), 15 cases were levofloxacin resistant (21.13%), of which 8 cases (11.27%) were dual resistant to both clarithromycin and levofloxacin. Besides, 33 cases were sensitive to clarithromycin and levofloxacin (47.14%). Conclusion: Both fluorescence quantitative PCR and immunohistochemistry have a good positive rate in the detection of Hp. Fluorescence quantitative PCR can not only detect Hp infection, but also reveal the drug resistance of anti Hp related antibiotics. PCR shows good rapidity and accuracy, with great clinical significance in guiding individualized treatment.