Objective: To study the influencing factors of cardiovascular autonomic dysfunction in patients with Parkinson’s disease (PD). Methods: Eighty patients with PD who were treated in the Department of Neurology of Port Hospital of Hebei Port Group Co., Ltd. from March 2018 to August 2019 were selected as the PD group, and 50 healthy volunteers in the same period were selected as the control group. The PD group underwent Hoehn-Yahr (H-Y) staging, Unified PD Rating Scale III (UPDRS III) score and Scale for Outcomes in PD for Autonomic Symptoms (SCOPA-AUT) score. All subjects were monitored by 24-hour ambulatory blood pressure and 24-hour ambulatory electrocardiogram, and the changes in blood pressure variability (BPV) and heart rate variability (HRV) indexes in the 2 groups were analyzed, the influencing factors of PD cardiovascular autonomic nerve dysfunction were explored, and multiple linear regression analysis was conducted. Results: The standard deviation of 24-hour systolic blood pressure (24h-SBPSD), standard deviation of daytime systolic blood pressure (dSBPSD), and standard deviation of systolic blood pressure at night (nSBPSD) of BPV indexes in the PD group were higher than those in the control group (all P<0.05). The differences in 24-hour average heart rate (24hHR), average heart rate at night (nHR) and average heart rate at daytime (dHR) between the 2 groups were statistically significant (all P<0.05). Standard deviation of all sinus R-R intervals within 24 hours (SDNN), percentage of adjacent NN intervals that differ from each other by more than 50 ms (pNN50%), root mean square value of all adjacent sinus R-R interval differences within 24 hours (RMSSD), high frequency component (HF), low frequency component (LF) and LF/HF in the PD group were significantly lower than those in the control group (all P<0.05). The nSBPSD index in PD group was positively correlated with the course of disease (r=0.301, P=0.020). SDNN index in the PD group was negatively correlated with H-Y staging, UPDRS III score and SCOPA-AUT score (r=−0.312, −0.356, −0.212, P=0.042, 0.023, 0.036); RMSSD index was negatively correlated with the course of disease (r=−0.345, P=0.026); LF/HF index was negatively correlated with H-Y staging (r=−0.213, P=0.046). Multiple linear regression analysis results showed that: 1) nSBPSD was independently correlated with the course of disease (P<0.05); 2) SDNN was independently associated with UPDRS III score and SCOPA-AUT score (P<0.05); 3) RMSSD was independently associated with the course of disease (P<0.05); 4) LF/HF was independently associated with H-Y stage (P<0.05). Conclusion: The increase of BPV and the decrease of HRV are the indicators reflecting the cardiovascular autonomic nerve dysfunction in PD patients, and the cardiovascular autonomic nerve dysfunction in PD patients is related to the duration of disease, the severity of disease, the severity of motor symptoms, and the dysfunction of autonomic nerve function regulation.