miR-124对C6胶质瘤细胞增殖和迁移能力的影响
| 作者: |
1王磊,
2张国庆,
3赵富花,
4左苗苗,
5曾现伟
1 潍坊医学院临床学院，山东 潍坊 261053 2 潍坊市高新区人民医院，山东 潍坊 261000 3 中国人民解放军95876部队医院中医内科，甘肃 张掖 734100 4 潍坊市人民医院脑科分院神经外科，山东 潍坊 261000 5 潍坊医学医院附属医院神经外科，山东 潍坊 261000 |
| 通讯: |
曾现伟
Email: zengxwei@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2017.02.028 |
| 基金: | 国家自然科学基金, 8167021239 国家科技部科技支撑计划, 2015BAI06B05 山东省科技发展计划项目, 2015GGB14296 |
摘要
目的：研究miR-124对C6胶质瘤细胞增殖和迁移能力的影响。方法：体外培养C6胶质瘤细胞，依据转染不同分为空白对照组、阴性对照组和miR-124拟似物组。实时荧光PCR检测转染效率，绘制生长曲线，MTT实验检测miR-124对C6细胞增殖能力影响，计算抑制率。划痕实验检测miR-124对C6细胞迁移能力影响，计算迁移率。结果：生长曲线显示miR-124拟似物组C6细胞生长能力受到明显抑制，转染后第3天，miR-124拟似物组C6细胞数目显著低于阴性对照组(5.410±0.463 vs. 6.917±0.385；P<0.01)；miR-124拟似物组mRNA表达量显著高于阴性对照组和空白对照组(5.92±0.56 vs. 0.93±0.13和1.00±0.12；P<0.01)；MTT增殖实验显示miR-124抑制C6细胞增殖能力，miR-124拟似物组抑制率显著高于阴性对照物组(42.90±5.169 vs. 10.24±3.351；P<0.01)；划痕实验显示miR-124明显抑制C6细胞迁移能力，阴性对照组迁移率显著高于miR-124拟似物组(98.79±1.210 vs. 81.72±5.972；P<0.05)。结论：miR-124能够显著抑制C6胶质瘤细胞的增殖和迁移能力。
关键词：
miR-124
C6胶质瘤细胞
增殖
迁移
Effect of miR-124 on proliferation and migration of C6 glioma cells
CorrespondingAuthor: ZENG Xianwei Email: zengxwei@163.com
DOI: 10.3978/j.issn.2095-6959.2017.02.028
Abstract
Objective: To investigate the effect of miR-124 on the proliferation and migration of glioma C6 cells in vitro. Methods: The glioma C6 cells cultured in vitro were divided into three groups as following an miR-124 mimic group, a negative control group and a blank control group. The expression level of miR-124 was detected by RT-PCR. Growth curves were drawn. MTT assay was used to determine the proliferation ability of C6 cells. The inhibition rate was calculated. Scratch migration assay was used to determine the migration ability of C6 cells. The mobility was calculated. Results: According to the growth curves, the number of glioma C6 cells in miR-124 mimic group was significantly less than those in the negative control group on the third day after transfection (5.410±0.463 vs. 6.917±0.385; P<0.01). Compared to the negative control group and the blank control group, the expression of miR-124 was higher in the miR-124 mimic group (5.92±0.56 vs. 0.93±0.13 and 1.00±0.12; P<0.01). According to MTT assay, miR-124 significantly inhibited the proliferation ability of C6 glioma cells, the inhibition rate in the miR-124 mimic group was significantly higher than that in the negative control group (42.90±5.169 vs. 10.24±3.351; P<0.01). According to Scratch migration assay, miR-124 inhibited the migration ability of C6 glioma cells, the migration rate in the negative control group was significantly higher than that in the miR-124 mimic group (98.79±1.210 vs. 81.72±5.972; P<0.05). Conclusion: MiR-124 can significantly inhibit the proliferation and migration of C6 glioma cells.