Objective: To investigate the effect of intraoperative warming blanket combined with isothermal lavage fluid in patients undergoing ureteroscopic holmium laser lithotripsy. Methods: A total of 94 patients with ureteroscopic holmium laser lithotripsy treated in Anhui Fuyang Second People’s Hospital from January 2021 to October 2021 were selected and randomly divided into a control group and a study group, 47 cases in each group by random number table method. The control group was given routine operating room intervention, and the study group was given warming blanket combined with isothermal lavage fluid for thermal insulation intervention. The general data and basic operation conditions of the two groups were recorded, focusing on the changes in body temperature, heart rate (HR) and mean arterial pressure (MAP) after anesthesia (T0), 30 minutes during operation (T1) and after operation (T2). The occurrence of perioperative hypothermia, chills and agitation in the two groups were recorded. Results: Compared with t0 time point of this group, the body temperature of T1–T2 in the two groups decreased significantly, and the HR and map increased significantly (P<0.05). The body temperature of T1–T2 in the study group was higher than that in the control group, and the HR and map were lower than those in the control group (P<0.05). There was no significant difference in the incidence of perioperative shivering and agitation between the two groups (P>0.05). The incidence of hypothermia in the study group was 10.64% and the total incidence was 14.89%, which were significantly lower than 29.79% and 40.43% in the control group (P<0.05). Conclusion: The intervention of warming blanket combined with other body temperature lavage fluid in ureteroscopic holmium laser lithotripsy is helpful to maintain the stability of body temperature, HR and map, and has a significant effect on reducing the occurrence of perioperative hypothermia, chills and agitation.