Objective: To investigate the application value of contrast-enhanced ultrasound in peripheral pulmonary consolidation biopsy. Methods: A total of 90 patients (90 lesions) with peripheral pulmonary lesions admitted to our hospital from January 2018 to December 2021 were selected. Among them, 48 patients underwent conventional ultrasound-guided needle biopsy were divided into a conventional ultrasound group and 42 patients underwent contrast-enhanced ultrasound-guided needle biopsy were divided into a contrast-enhanced ultrasound group. The puncture times, pathological diagnosis rate of puncture, necrotic area, and vascular display were compared between the 2 groups; the scores of interventional puncture path and needle tip display were compared between the 2 groups; and the enhancement phase characteristics of benign and malignant lesions in contrast-enhanced ultrasound group were compared. Results: Among the 48 patients who underwent routine ultrasound-guided lung puncture, 40 cases (83.33 %) were successfully pathological diagnosed (31 cases of malignant, 9 cases of benign), 8 cases (16.67%) were not successful diagnosed; among the 42 patients undergoing contrast-enhanced ultrasound-guided lung puncture, 41 (97.62%) were successfully punctured (34 malignant and 7 benign). The pathological diagnosis rate of puncture in the contrast-enhanced ultrasound group was significantly higher than that in the conventional ultrasound group (P<0.05), and there was no significant difference in puncture times between the 2 groups (P>0.05). The display rate of necrotic area and adjacent large vessels in the contrast-enhanced ultrasound group were significantly higher than those in the conventional ultrasound group (both P<0.05), and the maximum range of necrotic area was significantly larger than that in the conventional ultrasound group (P<0.05). There was significant difference in the scores of interventional puncture path and needle tip display between the 2 groups (both P<0.05), and the contrast-enhanced ultrasound group was superior to the conventional ultrasound group. In the contrast-enhanced ultrasound group, the enhancement time of malignant lesions was significantly earlier than that of benign lesions (P<0.05), while there was no significant difference in peak time and decline time between the 2 groups (both P>0.05). The incidence of complications in the conventional ultrasound group was 8.33%, and no complications occurred in the contrast-enhanced ultrasound group. There was no significant difference between the 2 groups (P>0.05). Conclusion: Contrast-enhanced ultrasound has obvious advantages over conventional ultrasound in the puncture biopsy of peripheral pulmonary consolidation, which can significantly improve the pathological diagnosis rate of puncture and help reduce the risk of puncture complications.