Objective: To investigate the expression of NOTUM gene in gastric cancer and to explore its clinicopathological relationship with gastric cancer. Methods: Eighty cases of gastric cancer tissues and normal gastric mucosa tissues were collected from October 2014 to April 2015 in General Surgery of the People’s Liberation Army General Hospital; The NOTUM gene expression of the gastric cancer tissues and the gastric mucosa tissues were used by the immunohistochemistry method, the relationship between the NOTUM gene expression and clinical pathologic parameter was analysed. The NOTUM protein and the β-catenin protein were detected by using the western blot method. Then the relationship of the NOTUM proteins and β-catenin proteins was analysed. The difference of the survival time was compared by the Kaplan-Meier method between the NOTUM positive expression and the NOTUM negative expression in postoperative patients with gastric cancer. Log-rank and Cox regression model were used to analyze the clinicopathological features that might affect the prognosis of patients. Results: The expression of NOTUM gene in gastric cancer tissues was significantly increased and it correlated with lymph node metastasis and TNM staging (P<0.05), it didn’t correlate with the patient’s age, sex, tumor location, tumor size and histological type. The expression of the NOTUM protein and β-catenin protein was positively correlated in gastric cancer tissues. The survival time in gastric cancer patients with the NOTUM gene highly expressed was significantly reduced. NOTUM gene expression might be the independent prognostic factor of the survival in patients with gastric cancer. Conclusion: NOTUM gene is highly expressed in gastric cancer tissues, and it may be used as a biological marker to predict the progression and prognosis of the gastric cancer.