Objective: To study the long-term clinical outcomes of donors with twin-to-twin transfusion syndrome after comprehensive nursing. Methods: Subjects were enrolled into the control group and the experimental group according to standard criteria, comprehensive nursing intervention to these donors was then carried out during the perinatal period, and the nursing effects of anemia, brain injury and other complications after 1 year out of hospital were compared with discharge point. Results: After comprehensive nursing care, red blood cells and hemoglobin content have no significant difference (P>0.05) between control group and experimental group at discharge point and after 1 year out of hospital; according to the results of “Bayley infant development scale” indicated that after follow-up recipients for 1 year, the distribution ratios at different levels of mental development index (MDI) and motor development index (PDI) in the donor twins have no significant difference (P>0.05) compare to the normal. Conclusion: Performing comprehensive nursing care to the donors of twin-to-twin transfusion syndrome in the perinatal period can effectively prevent the long-term complications.