Objective: To observe the effects of fast track surgery theory and early enteral nutrition on clinical indicators and immune function of patients with gastric cancer. To investigate the mechanisms and clinical value of promoting the rehabilitation of patients. Methods: A non-randomized controlled study was undertaken. Data were collected from 197 patients with gastric cancer who were treated from February 2010 to January 2014, including 92 patients having undergone FTS with EEN and 105 patients having undergone conventional perioperative care. Out comes were assessed using the time to first flatus and defecation, the length of postoperative hospital stay and postoperative complications. Furthermore, immunoglobulin and T lymphocyte subsets in blood samples from two group patients were detected. Results: The time to first flatus (2.2 vs. 4.9 d) and defecation (3.4 vs. 5.8 d) and the length of hospital stay (5.2 vs. 8.9 d) in the FTS group were significantly shorter than those in the conventional treatment group (all P<0.05). The level of IgA, IgM, CD4+,LYM and ratio CD4+/CD8+ in the FTS group were significantly higher than those in the conventional treatment group (all P<0.05). The level of CD8+was crosscurrent. The overall incidence of complications in the FTS group was lower than that in the conventional treatment group (28.2% vs. 29.5%), but the difference had no statistical significance (P=0.45). Conclusion: Application of FTS and EEN could promote recovery of bowel function of patients with gastric cancer and improve their immunologic function, however, it would not increase the incidence of postoperative complications.