Objective: To investigate the effects of different interventions on premature heel blood pain relief. Methods: Selected 156 premature infants in our hospital from July 2013 to December 2013, randomly divided into observation group A, observation group B, observation group C and control group. The observation group A, B, C was respectively treated with non-nutritive sucking, nutritional supplement, sucking touch intervention; the control group was taken no relief measures. Evaluated the puncture pain score of premature infants on the process of the heel prick and 5 min after that (based on premature infant pain scores short table evaluation), and the facial expression score, compared the scores in each group. Results: During the heel prick on the children in the three observation groups, PIPP scores were all lower than 6, which showed no need for analgesic treatment; 5 min after the puncture, the scores all decreased and the difference was significant when compared with the control group. The pain expression scores in the three observation groups were significantly lower than those in the control group; the scores in group B is nearly 0, which has the lowest expression score, also has better pain index and effects (P<0.05). Conclusion: Different methods of intervention on premature heel lancing can alleviate the pain, and nutritive sucking has better effects. We should choose more appropriate intervention measures according to the specific situation of the children in the clinic.