mTOR 和CCR7 与乳腺癌侵袭转移的关系
| 作者: |
1刘清华,
2李媛媛,
3徐滨,
4王竹青,
1刘雨清
1 潍坊医学院病理学教研室，山东 潍坊 261053 2 潍坊医学院病理学教研室，山东 潍坊 261053； 3 胜利油田中心医院病理科，山东 东营 257034 4 潍坊市中心医院病理科，山东 潍坊 261053 |
| 通讯: |
刘雨清
Email: yuqingliu89@hotmail.com |
| DOI: | 10.3978/j.issn.2095-6959.2013.02.001 |
| 基金: | 国家自然科学基金；山东省中青年科学家奖励基金, 81072068；2010BSB14050 |
摘要
目的：探讨乳腺浸润性导管癌组织中哺乳动物雷帕霉素靶蛋白( mammalian target of rapamycin，mTOR) 与趋化因子受体7 (chemokine receptor 7，CCR7) 蛋白的表达，以及二者与乳腺癌侵袭、转移之间的关系。方法：采用免疫组织化学方法检测65 例乳腺浸润性导管癌及32 例正常乳腺组织中mTOR 和CCR7 的表达情况，并分析二者之间的相关性及其与各临床病理特征之间的关系。结果：mTOR 和CCR7 蛋白在乳腺浸润性导管癌中的阳性表达率(mTOR 为68%，CCR7 为74%) 高于癌旁正常乳腺组织中的阳性表达率(mTOR 为25%，CCR7 为28%)，差异有统计学意义(P<0.01)。
mTOR 与CCR7 的表达呈正相关 (r=0.485，P<0.05)。mTOR 和CCR7 的高表达均与淋巴结转移、临床恶性肿瘤分期有关( 均P<0.05)，而与年龄、雌激素受体、孕激素受体无关( 均P>0.05)。结论：mTOR 和CCR7 的异常高表达可能与乳腺癌的侵袭、转移有关，且检测二者的表达可作为判断乳腺癌转移的预测指标。
关键词：
哺乳动物雷帕霉素靶蛋白；趋化因子受体7；乳腺浸润性导管癌；免疫组织化学；侵袭；转移
mTOR 与CCR7 的表达呈正相关 (r=0.485，P<0.05)。mTOR 和CCR7 的高表达均与淋巴结转移、临床恶性肿瘤分期有关( 均P<0.05)，而与年龄、雌激素受体、孕激素受体无关( 均P>0.05)。结论：mTOR 和CCR7 的异常高表达可能与乳腺癌的侵袭、转移有关，且检测二者的表达可作为判断乳腺癌转移的预测指标。
Relationship between mTOR, CCR 7 and the invasion and metastasis of breast carcinoma
CorrespondingAuthor: LIU Yuqing Email: yuqingliu89@hotmail.com
DOI: 10.3978/j.issn.2095-6959.2013.02.001
Abstract
Objective: To detect the expression of mammalian target of rapamycin (mTOR) and chemokine receptor chemokine receptor 7 (CCR7) protein in breast nfiltrating duct carcinoma, and to analyze the correlation between mTOR, CCR7 protein and the invasion and metastasis of breast cancer. Methods: Immunohistochemistry was used to detect the mTOR and CCR7 protein expression level in 65 patients with breast infiltrating duct carcinoma and 32 cases of normal breast tissue. At the same time, the relationship between the expression of mTOR, CCR7 and the clinicopathologic characteristics was analyzed. Results: The positive expression rates of the mTOR and CCR7 protein in the breast infiltrating duct carcinoma tissue (mTOR: 68%, CCR7: 74%) was significantly higher than those in the cancer adjacent tissue (mTOR: 25%, CCR: 7 28%)(P<0.01). The protein expression level of mTOR was positively related with that of CCR7 in breast infiltrating duct carcinoma tissue (r=0.485, P<0. 05) . The expression of mTOR and CCR7 were related with the lymph node metastasis and TNM stage (both P<0.05), but there is no correlation with the patients’ age, estrogen receptor, progesterone receptor (P>0.05). Conclusion:The abnormal expression of mTOR and CCR7 may be related with the occurrence, development, invasion and metastasis of the breast cancer, and the detection of mTOR and CCR7 protein expression may be served as the predict index for breast carcinoma metastasis.