新生儿缺氧缺血性脑病患儿血液中血管内皮生长因子和miR-125b表达水平及相关性研究
| 作者: |
1黄群,
1谭丹凤,
1马敏,
1黄广文,
1龙小兰,
1吴虹
1 湖南省妇幼保健院儿童保健科，长沙 410008 |
| 通讯: |
黄群
Email: huangqun613@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2014.04.007 |
摘要
目的：探讨血管内皮生长因子(vascular endothelial growth factor，VEGF)和miR-125b在新生儿 缺氧缺血性脑病(hypoxic-ischemic encephalopathy，HIE)患儿和正常新生儿血液中表达水平及 相关性。方法：收集20例正常新生儿和20例HIE患儿血液标本，分离血浆和外周血单个核细胞 (peripheral blood mononuclear cell，PBMC)，利用real-time PCR检测PBMC中miR-125b表达，ELISA 方法检测血浆中VEGF含量。结果：与正常对照组相比，HIE患儿PBMC中miR-125b表达显著降 低(P=0.00002)，而血浆中VEGF水平显著升高(P=6.26×10–8)；同时在正常组中，miR-125b表达与 VEGF水平无相关性(r=0.016，P=0.946，n=20)，但在HIE组(r=–0.505，P=0.023，n=20)二者却呈显 著负相关。结论：新生儿脑缺血缺氧时可能通过降低miR-125b表达从而升高VEGF表达。
关键词：
新生儿缺氧缺血脑病; 微小RNA; 血管内皮生长因子
Relationship between vascular endothelial growth factor and miR-125b in neonates with hypoxic-ischemic encephalopathy
CorrespondingAuthor: HUANG Qun Email: huangqun613@163.com
DOI: 10.3978/j.issn.2095-6959.2014.04.007
Abstract
Objective: To explore the expression and correlation between vascular endothelial growth factor (VEGF) and miR-125b in healthy and hypoxic-ischemic encephalopathy (HIE) neonatus. Methods: A total of 20 controls and 20 HIE samples were recruited in this study, plasma and peripheral blood mononuclear cell (PBMC) were prepared for detecting the miR-125b expression and VEGF concentration using real-time PCR and ELISA kit, respectively. Result: Compared with controls, miR-125b expression in PBMC was significantly decreased (P=0.00002) but VEGF concentration in plasma was increased in HIE group (P=6.26×10–8). Meanwhile, there was no significant correlation between miR-125b expression and VEGF level in controls (r=0.016, P=0.946,n=20). However, significant correlation was observed in HIE group (r=–0.505, P=0.023, n=20). Conclusion: The VEGF level was increased possibly due to the down-regulation of miR-125b in HIE neonatus.